Decreased Interleukin-4 Level of Type I Hypersensitive Mice Using Scopoloetin Isolated from Noni Fruit (Morinda citrifolia L.)
| Main Authors: | Aldi, Yufri, Yuliandra, Yori, Nasrul, Ellyza, Yanwirasti, Yanwirasti, Handayani, Dian, Bakhtiar, Amri |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Subjects: | |
| Online Access: |
http://repo.unand.ac.id/1672/1/%255B244%255D.pdf http://repo.unand.ac.id/1672/ |
Daftar Isi:
- An in vivo study of the activity of scopoletin isolated from noni fruit (Morinda citrifolia L.) on the level of interleukin-4 (IL-4) in type I hypersensitive male Swiss-Webster mice has been carried out. Scopoletin was isolated from dried noni powder by soxhletation method using dichloromethane, separated by column chromatography using silica gel as stationary phase and n-hexane-ethyl acetate (1:4) as mobile phase, then purified by column chromatography using Sephadex LH20 as stationary phase and methanol as mobile phase. Type I hypersensitive male mice were obtained by ovalbumin sensitization. Animal model were divided into 5 groups: negative control group, positive control group, and scopoletin-treated group (1; 3; and 10 mg/kg). The results showed that scopoletin at doses of 1, 3 and 10 mg/kg decreased the level of IL-4 of type I hypersensitive mice significantly (p <0.01). The scopoletin at the dose of 10 mg/kg decreased the serum level of IL-4 to the normal level.