Pengaruh Pemberian Madu terhadap Gambaran Basophilic Stippling Eritrosit Mencit (Mus musculus) yang Diberi Pb Asetat
| Main Author: | Winson |
|---|---|
| Other Authors: | Fitrie, Alya Amila |
| Format: | Student Papers |
| Bahasa: | ind |
| Subjects: | |
| Online Access: |
http://repository.usu.ac.id/handle/123456789/31314 |
Daftar Isi:
- Background : Lead (Pb) in the blood at a certain level can cause health problems in various human organs, including hematopoetic system. Pb at the toxic level causes the G-6PD deficiency and the inhibition of pirimidine-5’-nucleotidase so the RNA and ribosome become acumulated marked with the finding of the basophilic stippling erythrocytes. The toxicity of Pb can be overcome by the administeration of honey which has been proved to contain various compositions of strong antioxidant. Objective : to find out the affect of the honey to the presentation of the mice’s basophilic-stippling erythrocyte after exposed by lead. Methods : The design used in this study was experimental with three groups of male mice (Mus musculus). The population of this study was 6-8 week old male mice weighing 30-50gr and healthy that was marked with an active movement and then devided into 3 groups. Groups I (control) was given aquadest. Group II was given lead acetate 100mg/kgBW/day orally using a gavage needle. Group III was given lead acetate 100mg/kgBW/day and honey 0,04 mL of honey/20 gram mice/day diluted to 0,2 cc at the same time. After 8 weeks, samples were aspirated some of their blood from their tail’s vein and made into blood sample. Then, this blood sample were directly made into the blood smear. The sum of the basophilic stippling erythrocytes were counted under the microscope. Data were collected and analyzed with Mean-Whitney test. Results: The result of this research showed that there were basophilic stippling erythrocytes in the K2 and K3 groups. The stastical analysis utilizing the Kruskal- Wallis Test followed with Mean-Whitney Test showed that there was a significant difference between the K2 and K3 groups (p<0.001). Conclusion: From the result of this experimental research, can be concluded that Honey can reduce the adverse effect of the lead toxicity.
- 080100108