Effect of Xylitol on Candida albicans resistance in serum (in vitro study)
| Main Authors: | Puspitawati, Ria; Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Kadrianto, Theodorus Hedwin; Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Bachtiar, Boy M.; Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Leepel, Lakshmi A.; Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430 |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Dentistry, University of Indonesia
, 2013
|
| Subjects: | |
| Online Access: |
http://www.jdentistry.ui.ac.id/index.php/JDI/article/view/98 http://www.jdentistry.ui.ac.id/index.php/JDI/article/view/98/91 |
Daftar Isi:
- Xylitol is reported to inhibit the growth of C. albicans. Objectives: Investigating serum factor role in inhibiting the growth of C. albicans and the effect of 1%, 5%, 10% xylitol on C. albicans resistance in serum in vitro. Methods: Identification of C. albicans (oral swab of candidiasis patient) was conducted using CHROMAgar, confirmed by germ tube test. The cultures were serially diluted, inoculated in Saburoud Dextrose Broth (SDB) contained 0% (control), 1%, 5%, or 10% xylitol, and kept for 3 or 7 days. These inoculations were then exposed to either active or inactive serum (Fetal Bovine Serum heated in 65oC for 30 minutes) for 2 hours in 37oC. The colony forming unit (CFU) of C. albicans in Saburoud Dextrose Agar (SDA) were counted after 2 days. C. albicans ATCC 10231 strain was used as a comparison. One-way ANOVA with 0.05 was used. Results: After 3 days cultured in media with or without xylitol, the CFU of C. albicans exposed to active serum were significantly lower than those exposed to inactive serum (p=0.032). Although not statistically significant (p=0.689), increased concentration of xylitol lead to increased resistance of C. albicans in active serum. Only 7 day exposure of 10% xylitol resulted in significantly higher growth of C. albicans (p=0.034). No significant difference of C. albicans CFU in active or inactive serum (p=0.404). Conclusion: Serum factor has role in inhibiting C. albicans growth in vitro. Exposure of 1%, 5%, or 10% xylitol for 3 or 7 days has no significant effect on C. albicans resistance in serum.DOI: 10.14693/jdi.v16i2.98