Efek Penambahan Glukosa pada Saburoud Dextrose Broth terhadap Pertumbuhan Candida albicans (Uji In Vitro)
| Main Authors: | Leepel, Lakshmi A.; Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Hidayat, Rahmat; Undergraduate Program, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Puspitawati, Ria; Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Bahtiar, Boy M; Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430 |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Dentistry, University of Indonesia
, 2012
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| Subjects: | |
| Online Access: |
http://www.jdentistry.ui.ac.id/index.php/JDI/article/view/14 http://www.jdentistry.ui.ac.id/index.php/JDI/article/view/14/11 |
Daftar Isi:
- High carbohydrate intake is one of predisposing factors of oral candidiasis. Objective: Investigating the effect of 1%,5%,10% glucose addition on the growth of C.albicans in vitro. Method: C.albicans sample was taken from oral swab of a male oral candidiasis patient. Identification of C.albicans was conducted using CHROMagar and confirmed by germ tube formation in serum. As a comparison, C.albicans ATCC10231 was used. After 2 days the cultures were serially diluted and inoculated in SDB without glucose, and with 1%,5%,10% addditional glucose, kept for 3 and 7 days in room temperature, then inoculated in SDA. The CFU/ml were counted after 2 days. ANOVA with α0.05 was used. Result: Statisticaly, additional 1% glucose for 3 days lead to significant decreased of growth of both clinical strain and ATCC 10231 C. albicans. However, only additional 5% and 10% glucose in clinical isolate for 7 days increased the growth of C.albicans significantly. Conclusion: The effect of additional glucose on the increased growth of C.albicans in vitro is influenced by the concentration, exposure duration of glucose, and by the strain of C.albicans.DOI: 10.14693/jdi.v16i1.14