Molecular taxonomy via DNA barcodes for species identification in selected genera of Fabaceae
| Main Authors: | Wardani, I Gusti Ayu Kusuma, Amandita, Fitri Yola, Moura, Carina Carneiro de Melo, Gailing, Oliver, Siregar, Iskandar Z |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Graduate School Bogor Agricultural University (SPs IPB)
, 2022
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jpsl/article/view/36609 http://journal.ipb.ac.id/index.php/jpsl/article/view/36609/23336 |
Daftar Isi:
- Fabaceae merupakan famili yang penting secara ekologi dan ekonomi karena manfaatnya yang tinggi sebagai sumber makanan, pupuk hijau, serta tanaman obat. Namun, di Indonesia Fabaceae telah dieksploitasi secara berlebihan di Indonesia dan beberapa spesies termasuk dalam kategori terancam punah. Untuk mendukung upaya konservasi dibutuhkan teknik identifikasi yang cepat dan akurat. Saat ini, identifikasi spesies melalui DNA barcode telah menjadi teknik klasifikasi taksonomi yang efektif. Penelitian ini bertujuan untuk mengevaluasi efektivitas penanda matK, rbcL, serta kombinasinya (matK+rbcL), sebagai DNA barcode dalam identifikasi spesies dari famili Fabaceae. Hasil penelitian menunjukkan bahwa matK+rbcL dan matK memiliki tingkat identifikasi tertinggi (90% dan 82,05%) pada tingkat spesies. Selain itu, matK memiliki rata-rata jarak interspesifik tertinggi (0.314) dan jarak intraspesifik (0.0030). Selanjutnya pohon filogenetik terbaik dihasilkan dengan metode Neighbour Joining. Berdasarkan analisis secara keseluruhan matK dan matK+rbcL merupakan barcode terbaik untuk spesies terpilih dalam penelitian ini.
- Fabaceae is an invaluable plant family with considerable ecological and economic importances for example as food sources, bio-fertilizer, and medicinal plants. However, Fabaceae has been overexploited in Indonesia and several species belong to this family are critically endangered. Due to morphological similarity, rapid and accurate identification of Fabaceae species is essential to support its conservation efforts. Nowadays, species identification through DNA barcoding has become an effective taxonomic classification tool. This study was aimed to evaluate the effectiveness of chloroplast markers, matK and rbcL and the combination (matK+rbcL), as DNA barcodes for the identification of seleted genera in Fabaceae. The result showed that matK+rbcL and matK had the highest level of identification (90% and 82.05%) of the investigated genera, respectively. Additionally, matK had the highest mean of interspesific distance (0.134) and intraspecific distance (0.003). The combined barcode matK+rbcL had the highest correct identification rate when comparing the morphological with molecular identification. Furthermore, the best phylogenetic tree was obtained using Neighbor Joining method. Based on the overall performance, matK and matK+rbcL were the best barcodes for the selected genera in this study.