Effect of B-carotene on Cell Proliferation and Differentiation of Adipose-derived Stem Cells into Endothelial Progenitor Cells
| Main Authors: | Widowati, Wahyu, Sardjono, Caroline Tan, Wijaya, Laura, Laksmitawati, Dian R. , Pawitan, Jeanne Adiwinata, Sandra, Ferry |
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| Format: | Article PeerReviewed Book |
| Terbitan: |
, 2014
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| Subjects: | |
| Online Access: |
http://repository.maranatha.edu/5288/1/Ereprint_BAIJ_858%20%28Widowati-FKUKM%29.pdf http://repository.maranatha.edu/5288/ |
Daftar Isi:
- Aim: To evaluate the ability of â-carotene to protect oxidation effect of H2O2, and to enhance proliferation and differentiation into AD-MSCs into EPCs. Experimental: AD-MSCs were induced by EGM Bullet Kit medium with or without â-carotene. The proliferation of EPCs was determined by viable cell number counts. Differentiation into EPCs was characterized by the following surface markers: CD 34, CD133 and vascular endothelial growth factor receptor 2 (VEGFR-2). The protective effect of â-carotene was measured base on the level of intracellular reactive oxygen species (ROS) by fluorescence with 2�,7�- dichlorofluorescein diacetate (DCF-DA) using flow cytometry. Results: â-Carotene enhanced cell proliferation and differentiation of AD-MSCs into EPCs, and also decreased the accumulation of H202-induced intracellular ROS in EPCs. Conclusion: â- Carotene plays a role in cell proliferation and differentiation of AD-MSCs to EPCs that might be due to decreased intracellular ROS level.