Turmeric Extract Potential Inhibit Inflammatory Marker in LPS-Stimulated Marcophage Cells
| Main Authors: | Widowati, Wahyu, Jasaputra, Diana Krisanti, Gunawan, Kamila Yashfa, Kusuma, Hanna Sari Widya, Arumwardana, Seila, Wahyuni, Cintani Dewi, Lister, I Nyoman Ehrich, Ginting, Chrismis Novalinda, Girsang, Ermi, Rizal, Rizal |
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| Format: | Article PeerReviewed Book |
| Terbitan: |
, 2021
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| Subjects: | |
| Online Access: |
http://repository.maranatha.edu/30177/1/Int%20Proceeding%202021-4%20%28Wahyu%20IEEE-Wahyu%20FKUKM%29.pdf http://repository.maranatha.edu/30177/ |
Daftar Isi:
- Objective: Inflammation can be induced by microbiological, chemical, physical factors and plays roles in inflammatory diseases. Turmeric (Curcuma longa L.) has been widely used to provide a diverse array of biological activities, including anti-inflammatory, antimicrobial, also antioxidant. The Turmeric extract (TE) anti-inflammatory potential was conducted using a Lipopolysaccharide (LPS)-induced RAW264.7 macrophage cell line by inhibiting inflammatory mediators especially IL-6, PGE-2, IL-1β, COX-2, TNF-α, iNOS, also NO level. Methods: The TE safe concentration in LPS-induced macrophage cell line was measured using MTS assay for further assay. The inflammatory markers (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO) were measured using ELISA assay and NO by the nitrate/nitrite colorimetric assay in LPSinduced RAW264.7 cell line. LPS induced inflammatory marker by increasing inflammatory marker (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO). Results: TE with 100 to 25 μg/ml, caused a significant reduction of cells viability, reaching only 30.27 % live cells. TE with lower concentrations (7.5; 5; 2.5 μg/ml) had no cytotoxic effect on macrophage cells (viability 117.31-131.08 %). LPS induction caused an increase in inflammatory cytokines IL-1β, PGE-2, IL-6, COX-2, TNF-α as well as iNOS and NO. Turmeric extract caused the reduction of the inflammatory cytokines in a dosedependent manner. Conclusion: The research resulted that TE has anti-inflammatory activity by decreasing IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, and NO level on LPS-induced RAW264.7 cells.