Daftar Isi: Ethanol Extract of Detam 1 Soybean Seed (Glycine Max L. Merr) for Chronic Kidney Disease Therapy by In Vitro Study

Main Authors:	Prahastuti, Sijani, Hidayat, Meilinah, Hasiana, Stella Tinia, Widowati, Wahyu, Amalia, Annisa , Qodariah, Rismawati Laila , Rizal, Rizal, Wibowo, Satrio Haryo Benowo, Kusuma, Hanna Sari W.
Format:	Article PeerReviewed Book
Terbitan:	Faculty of Pharmacy Universitas Gajah Mada , 2019
Subjects:	R Medicine (General)
Online Access:	http://repository.maranatha.edu/27830/3/63.Sinta2%202019-1%20%28Sijani%20TMJ%20-%20Wahyu%20FKUKM%29%20Reviewer.pdf http://repository.maranatha.edu/27830/1/63.%20Turnitin_Ethanol%20Extract%20of%20Detam%201%20Soybean%20Seed%20%28Glycine%20Max%20L.%20Merr%29%20for%20Chronic%20Kidney%20Disease%20Therapy%20by%20In%20Vitro%20Study.pdf http://repository.maranatha.edu/27830/2/63.Sinta2%202019-1%20%28Wahyu%20TMJ-Wahyu%20FKUKM%29%20Corr%20Author.pdf http://repository.maranatha.edu/27830/

Daftar Isi:

Chronic Kidney Disease (CKD) has increased incidence and prevalence in developing nations. In this in vitro study, we evaluated the cells proliferative effects, fibronectin (FN), transforming growth factor β (TGF-β1), and Reactive oxygen species (ROS) - level inhibition potential of ethanol extract of detam 1 soybean seed (EEDS) on glucose-induced kidney mesangial cells (SV40 MES 13). The cells proliferation assay used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium (MTS) assay. FN and TGF-β1 level were measured using ELISA assay kit and ROS level using flow cytometry. Level of FN, TGF-β1 and ROS, on CKD cells model (5 mM, 10 mM glucose-induced mesangial cell) treated with EEDS 6.25 μg/mL were lower significantly compared to positive control, EEDS improve cells viability and decrease FN, TGF-β1 and ROS level in glucose-induced kidney mesangial cells as CKD cells model. Keywords: chronic kidney disease; fibronectin; ROS; soybean; TGF-β1