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Conditioned medium of IGF1-induced synovial membrane mesenchymal stem cells increases chondrogenic and chondro protective markers in chondrocyte inflammation

Main Authors: Marlina, Marlina, Armenia, Armenia, Aviani, Jennifer Kiem, Sholihah, Ika Adhani, Kusuma, Hanna Sari W. , Azizah, Alya Mardhotillah, Elida, Nur, Widowati, Wahyu
Format: Article PeerReviewed Book
Terbitan: , 2021
Subjects:
R Medicine (General)
Online Access: http://repository.maranatha.edu/27823/2/62.Int%20Publikasi%20%202021-5%20%28Wahyu%20BioSciR%20-%20Wahyu%20FKUKM%29%20Reviewer.pdf
http://repository.maranatha.edu/27823/3/62.%20Turnitin_Conditioned%20Medium%20of%20IGF1-Induced%20Synovial%20Membrane.pdf
http://repository.maranatha.edu/27823/1/62.Int%20Publikasi%20%202021-5%20%28Wahyu%20BioSciR-Wahyu%20FKUKM%29%20Corr%20Author.pdf
http://repository.maranatha.edu/27823/
Daftar Isi:
  • Recently,mesenchymalstemcells(MSCs)have been the most explored cells for cell therapy for osteoarthritis(OA) that can be obtained from various sources.Synovial membrane MSCs (SMMSCs) provide bestpotentialforOAtherapy,howevertheyarenotwidelyexplored.Con- ditioned mediumofSMMSCs(SMMSCs-CM)richingrowthfactorsandcytokinescaninhibit apoptosis andincreasechondrocytescellproliferation.Theaimofthepresentstudywasto determine growthfactorscontentinSMMSCs-CMaswellasthechondrogenicandchon- droprotectivemarkersexpressioninOAmodelafterinsulin-likegrowthfactor(IGF)1-induced and non-inducedSMMSCs-CMtreatments.Chondrocytecellline(CHON002)wasinduced by IL1β as OAmodel(CHON002withIL1β (IL1β-CHON002)) andtreatedwithSMMSCs-CM with orwithoutIGF1inductiontodetermineitseffectivenessinrepairingOAcellsmodel. ELISA wasusedtoassayBMP2,fibroblastgrowthfactor18(FGF18)andtransforming growthfactor(TGF) β1 (TGFβ1) levelsinSMMSCs-CM,matrixmetalloproteinase(MMP)13 (MMP13) andadisintegrinandmetalloproteinasewiththrombospondinmotif4(ADAMTS4) levels inOAcellsmodeltreatedwithSMMSCs-CM.RT-qPCRanalyseswereusedtoinves- tigate thegeneexpressionofSOX9,COL2,andCOL10.CMfromSMMSCsculturedand induced byIGF1150ng/mLwasthemosteffectiveconcentrationforincreasingthecontent of growthfactormarkersofSMMSCs-CM,whichhadsuccessfullyincreasednegativecar- tilage hypertrophymarkers(SOX9andCOL2)andreducedhypertrophymarkers(COL10, MMP13, andADAMTS4).PreconditioningwithIGF1hasbetterandverysignificantresults in loweringMMP13andADAMTS4levels.ThepresentstudysupportsIGF1pre-conditioned SMMSCs-CM todevelopanewtherapeuticapproachinOAimprovementthroughitschon- drogenicandchondroprotectiveroles.

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