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Cosmeceutical potentials of Curcuma mangga Val. extract in human BJ fibroblasts against MMP1, MMP3, and MMP13

Main Authors: Pujimulyani, Dwiyati, Suryani, Ch. Lilis, Setyowati, Astuti, Handayani, Rr. Anisa Siwianti, Arumwardana, Seila, Widowati, Wahyu, Maruf, Ali
Format: Article PeerReviewed Book
Terbitan: , 2020
Subjects:
R Medicine (General)
Online Access: http://repository.maranatha.edu/27813/3/50.Int%20Publiaksi%202020-6%20%28Wahyu%20Heliyon-Wahyu%20FKUKM%29%20Reviewer.pdf
http://repository.maranatha.edu/27813/1/49.%20Cosmeceutical%20potentials%20of%20Curcuma%20mangga%20Val.%20extract%20in%20human%20BJ%20%EF%AC%81broblasts%20against%20MMP1%2C%20MMP3%2C%20and%20MMP13.pdf
http://repository.maranatha.edu/27813/
Daftar Isi:
  • Oxidativestress,thedisruptedoxidation-reductionmechanisminourbody,iscausedbytheexcessiveexposureof free radicalsandtheimpairedantioxidantdefensesthatcanaccelerateskinaging.Antioxidantscanbeobtained from nature,whichareavailablewidelyintherapeutic-richplants,suchaswhitesaffron(Curcuma mangga Val., denotedas C. mangga). Althoughmanypiecesofevidencerevealthat C. mangga contains anabundanceof phenoliccompoundsandhasantioxidativeeffects,itscosmeceuticalpotentialsremainunclear.Thepresentstudy aimed todisclosetheunexploredantiagingpotentialsof C. mangga extract (CME)inoxidativestress-induced human BJ fibroblasts withafocusoncollagenprotectionagainstpro-inflammatory mediatorsMMP1,MMP3, and MMP13.Theoxidativestress-inducedcellsweretreatedwithCMEandcurcuminatdifferentdoses.The resultsshowedthattreatmentusingCME(25 μg/mL) couldmaintainthecollagencontentsupto18.45 � 0.68 μg/ mL inH2O2-treated fibroblasts (only~26.63%reductionincollagencontents),whilethe figure forthenegative control wasthelowest(12.79 μg/mL), showingasignificant reductionincollagencontentsby49.13%.In addition,thegeneexpressionofpro-inflammatory MMPsarosesignificantly inBJ fibroblasts afteroxidativestress inductionusing200 μM H2O2, inwhichtheexpressionforMMP1,MMP3,andMMP13increasedby7.10,38.96, and 2.69times,respectively.Interestingly,CMEtreatment(100 μg/mL) couldeffectivelyinhibitMMP1,MMP3, and MMP13geneexpressionby3.65,34.62,and2.02times,respectively.Inconclusion,CMEshowedfavorable antiagingactivitiesinH2O2-treated humanBJ fibroblasts asconfirmed bythelowlevelsofgeneexpressionof MPP1, MMP3,andMMP13aftertreatmentwithCME.

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