Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (CM-hATMSCs)
| Main Authors: | Noverina, Rachmawati, Widowati, Wahyu, Ayuningtyas, Wireni, Kurniawan, Dedy, Afifah, Ervi, Laksmitawati, Dian R. , Rinendyaputri, Ratih, Rilianawati, Rilianawati, Faried, Ahmad, Bachtiar, Indra, Wirakusumah, Firman Fuad |
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| Format: | Article PeerReviewed Book |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
http://repository.maranatha.edu/27381/1/Int%20Publikasi%202019-3%20%28Noverina-Clynical-Wahyu%20FKUKM%29.pdf http://repository.maranatha.edu/27381/ |
Daftar Isi:
- Background: Stem cell-derived by conditioned medium has potential as regenerative agent of skin cells because it contains many growth factors, cytokines and other regenerative biomolecules. Conditioned medium (CM) from human adipose tissue-derived MSCs (CM-hATMSCs) also has been known enriched growth factors that play an important role in epithelial wound repair, reduce wrinkles, and enhance wound healing. Objective: This study was performed to evaluate growth factors in CM such as Transforming Growth Factor (TGF)-b1, TGF-b2,Vascular Endothelial Growth Factor (VEGF)-2, Vascular Cell Adhesion Molecule (VCAM) 1, Epidermal Growth Factor (EGF), Fibroblast Growth Factor (FGF) in CM-hATMSCs treated with Fresh Frozen Plasma (FFP) and non-FFP at various passages. Methods: This study used ELISA method to measure the growth factors in CM-hATMSCs. Results: FGF was the highest modulator among FFP and non-FFPtreated cells at many passages compared to the others growth factor. Both FFP and non-FFP-treated cells showed significant difference (P < 0.05) in some growth factors. However, there was no significant differences in TGF-b1 at passage 3,7,11 and 15 in FFPtreated cells and non-FFP-treated cells. Conclusion: In summary, the highest concentration level found in CM-hATMSCs is FGF both in FFP and non-FFP-treated cells. FGF as growth factor composition in CM-hATMSCs has potential as wound healing and regeneration of cell and it can be used in antiaging products.