The G6PDH Inhibition Activity Effects of Ethanol Extract of Detam 1 soybean, Jati Belanda leaves and Their Combinations in 3T3-Ll Culture Cells
| Main Authors: | Hidayat, Meilinah, Soeng, Sylvia, Prahastuti, Sijani |
|---|---|
| Format: | Proceeding PeerReviewed Book |
| Terbitan: |
, 2014
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| Subjects: | |
| Online Access: |
http://repository.maranatha.edu/27092/1/The%20G6PDH%20Inhibition%20Activity%20Effects%20of%20Ethanol%20Extract%20of%20Detam%201%20soybean%2C%20Jati%20Belanda%20leaves%20and%20Their%20Combinations%20in%203T3-L1%20Culture%20Cells.pdf http://repository.maranatha.edu/27092/ |
Daftar Isi:
- The prevalence of obesity is definitely increasing in all over the world. In obese subjects it was found that serum concentration of Glucose -6-phosphate dehydrogenase (G6PDH) increased significantly with increasing body weight. There are many Indonesian herbs have good effects in weight loss, for examples, Detam 1 soybean from Balitkabi Malang and Jati Belanda from BHD plantation . In this study, we examined the ethanol extract of Detam 1 soybean seed (EEDS), Jati Belanda leaves (EEJB) and their combinations (EESJB). The purposes of this study are to determine the safety of these extracts, and their effects to GPDH inhibition activity in 3T3-L l culture cells. Method: the effects of 3 concentration (lOOug/ml, 50 μg/ml, 10 μg/ml) of each samples to viability 3T3-Ll cells were measured triplo using cell titer 96 MTS Assay and GPDH inhibition activity effects to 3T3-L l cells using G6PDH kit, Abnova Cat KA 0880 with calorimetric method, counted at minutes 0 and 30. Average results of viability cells which treated with EEDS lOOug/ml: 85.39; 50 μg/ml: 93.79; 10 μg/ml: 96.59. With EEJB 81.77; 90.79; 93.52. With EESJB 76.66; 81.64; 88.60. The samples which viability > 90.0 was proved to be safe, so we only continued EEDS and EEJB 50 Jig/ml, 10 μg/ml. Average results of G6PDH inhibition activity: EEDS 50 μg/ml: 19.32 , 10 μg/ml: 11.91. EEJB 51.48 ; 20.12. Conclusion: The EEDS and EEJB 50 μg/ml, 10 μg/ml is proved to be safe to cells and EEJB 50 μg/ml has the best effect in G6PDH inhibition activity .