Cytotoxic Activity of Detam 1 Soybean and Jati Belanda (Guazuma ulmifolia) Extracts on SV40 MES 13 Cell
| Main Authors: | Hidayat, Meilinah, Prahatusti, Sijani, Soemardji, Andreanus A., Rihibiha, Dwi Davidson, Afni, Merry, Afifah, Ervi, Widowati, Wahyu |
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| Format: | Proceeding PeerReviewed Book |
| Terbitan: |
, 2016
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| Subjects: | |
| Online Access: |
http://repository.maranatha.edu/27091/1/Cytotoxic%20Activity%20of%20Detam%201%20Soybean%20and%20Jati%20Belanda%20%28Guazuma%20ulmifolia%29%20Extracts%20on%20SV40%20MES%2013%20Cell.pdf http://repository.maranatha.edu/27091/ |
Daftar Isi:
- The amount of Diabetes mellitus patients in Indonesia is predicted to be continually increasing. Lifestyle and pancreatic dysfunction in producing insulin, have been known as the main causes. Furthermore, diabetes mellitus type 1 and 2 can progressively develop, resulting in complication known as “diabetic glomerulosclerosis”. Diminished glomerular basement membrane causes failure in urine filltration, that causes presence of albumin in urine. Natural sources in Indonesia has been reported to contain bioactive compounds that possess medicinal properties. Jati Belanda (Guazuma ulmifolia) and Detam 1 soybean has been known to act as antiobesity and antidiabetes due to presence of its secondary metabolite. Objectives: This study aimed to determine cytotoxic activity of extract of Detam 1 soybean, Jati Belanda, and combination of extracts, toward SV40 MES 13 cell (Glomerular Mesangial Kidney, Mus musculus). Methods: Cytotoxic activity was measured with 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay. Concentrations used were 3.125, 6.25, 12.5, 25, 50, and 100 μg/mL. Results: Both Jati Belanda and Detam 1 soybean extract at concentration of 3.125, 6.25, and 12.5 μg/mL showed no toxicity toward SV40 MES 13 cell, as indicated by >90% viable cells, whilst at concentration of 25, 50, and 100 μg/mL showed toxicity, as indicated by <90% viable cells. The combination of extracts showed no toxicity only at low concentration (3.125 μg/mL). Conclusion: Thus, Jati Belanda and Detam 1 soybean extract at concentration of 3.125, 6.25, 12.5 μg/mL and the combination of extracts at 3.125 μg/mL were considered safe toward SV40 MES 13 cell.