Modulation of Adipogenesis-Related Gene Expression by Ethanol Extracts of Detam 1 Soybean and Jati Belanda Leaf in 3T3-L1 Cells
| Main Authors: | Hidayat, Meilinah, Prahastuti, Sijani, Fauziah, Nurul, Maesaroh, Maesaroh, Balqis, Balqis, Widowati, Wahyu |
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| Format: | Article PeerReviewed Book |
| Terbitan: |
, 2016
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| Subjects: | |
| Online Access: |
http://repository.maranatha.edu/20716/1/Int%20Publikasi%202016-3%20%28BJP%20Mella-Wahyu-FKUKM%29.pdf http://repository.maranatha.edu/20716/ |
Daftar Isi:
- In this study, we evaluated the effects of ethanol extracts of Detam 1 soybean, Jati belanda leaf, and the combination toward expression of peroxisome proliferator-activated receptor gamma (PPAR), CCAAT/enhancer-binding protein alpha (C/EBP), and stearoyl-CoA desaturase 1 (SCD1) genes in 3T3- L1 cells as anti-adipogenesis and anti-obesity. The differentiation of 3T3-L1 cells into adipocyte was conducted using induction medium consist of Dulbecco's Modified Eagle's Medium, 3-isobutyl-1-methylxanthine, insulin, dexamethasone, and fetal bovine serum. The expression of PPAR, C/EBP, and SCD1 gene was measured using real-time quantitative polymerase chain reaction (qPCR). Ethanol extract of Jati belanda at a concentration of 50 μg/ mL was most effective to reduce PPAR, C/EBP, and SCD1 gene expression in 3T3-L1 cells. Ethanol extract of Detam 1 soybean failed to reduce PPAR gene expression, whilst in the concentration of 50 μg/mL it was able to significantly reduce the C/EBP and SCD1 gene expression. Both ethanol extracts of Detam 1 soybean and Jati belanda have potential as antiadipogenesis and anti-obesity by suppressing adipogenesis-related gene expression, particularly C/EBP and SCD1.