Phytochemical Assay, Potential of Antimalarial and Antioxidant activities of Green Tea Extract and Its Fraction
| Main Authors: | Rahardja, Fanny, Tjokropranoto, Rita, Widowati, Wahyu, Lusiana, Siska, Suhendra, Adrian, Tjahjani, Susy, Budiman, Iwan, Maesaroh, Maesaroh, Fauziah, Nurul |
|---|---|
| Format: | Article PeerReviewed Book |
| Terbitan: |
, 2015
|
| Subjects: | |
| Online Access: |
http://repository.maranatha.edu/15161/1/Green%20Tea%20%28Fanny-2015%29.pdf http://repository.maranatha.edu/15161/ |
Daftar Isi:
- Objective : The research objective was to evaluate the phytochemical compounds, the antioxidant and anti-malarial activity of green tea extract and its fraction. Methods : The antioxidant activity were measured by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity used in vitro laboratories prospective experimental design and completely randomized design, using ethanol extract, hexane, ethyl acetate, butanol fraction, and water fraction of green tea in 7 levels of concentration compared to epicatechin (EC). The data were analyzed using linear regression analysis, and were continued by determined Inhibitory Concentration50 (IC50). The anti-malarial activity of green tea fractions were compared to artemisinin. The data were analyzed using probit analysis, and were continued by determined IC50 . Result: The results showed that the highest antioxidant activity (IC50) was the fraction of ethyl acetate 2,184 μg/ml. The anti-malarial activity IC50of water and ethyl acetate fraction, ethanol extract and butanol fraction were 0,000090 μg/mL,0,003 μg/mL, 0,006 μg/mL, 0,011 μg/ml respectively.