Ex Vivo Study of Garcinia Mangostana L. (Mangosteen) Peel Extract and Xanthones as Anti-Adipogenesis in HEPG2 Cell Model
| Main Authors: | Darsono, Lusiana, Hidayat, Meilinah, Maesaroh, Maesaroh, Fauziah, Nurul, Widowati, Wahyu |
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| Format: | Article PeerReviewed Book |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
http://repository.maranatha.edu/14524/1/11.%20Ex%20Vivo%20Study%20of%20Garcinia%20Mangostana%20L%20%28Mangosteen%29%20Peel%20Extract.pdf http://repository.maranatha.edu/14524/ |
Daftar Isi:
- Background: Anti-adipogenesis is one of proposed mechanism for anti-obesity. Adipogenesis regulation of obesity is important, so identification of anti-adipogenic activity is a potential strategy to find anti-obesity agent. Aim: The aim of this study is to evaluate the anti-adipogenesis potential of Garcinia mangostana L. peel extract (GMPE) compared to xanthones in HepG2 cells line as model. Material and Methods: GMPE was performed based on maceration method using distilated ethanol 70% as the solvent. The level of triglyceride and cholesterol and the inhibitory activity of triglyceride (TG) and cholesterol (CHOL) in HepG2 cells were assayed and determined as the anti-adipogenesis parameter. Results: The most active subtance to lower the triglyceride level was showed by GMPE in every concentration followed by the garcinone-C, γ-mangostin, garcinone-D and α- mangostin respectivelly. The highest activity to decrease the cholesterol level was showed by GMPE and followed by γ-mangostin, α-mangostin, garcinone-c, garcinone-d respectively. Conclusion: GMPE posses the anti-adipogenesis potential in inhibiting TG and CHOL synthesis was better than any other xanthone (α- mangostin, γ-mangostin, garcinone-C and garcinone-D).