INDUKSI DAN MULTIPLIKASI KALUS DARI JARINGAN ENDOSPERMA SIRSAK (Annona muricata L.) SECARA IN VITRO
| Main Author: | MA’RIFAT, MILA |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://eprints.untirta.ac.id/9526/1/INDUKSI%20DAN%20MULTIPLIKASI%20KALUS%20DARI%20JARINGAN%20ENDOSPERMA%20SIRSAK%20%28Annona%20muricata%20L.%29%20SECARA%20IN%20VITRO.PDF https://eprints.untirta.ac.id/9526/ |
Daftar Isi:
- Endosperm tissue culture is one of method to produce triploid plants. In the process, the application of growth regulating substances was needed in the success of tissue culture. This research aims to determine the concentrations of 2,4-D growth regulators, tomato extract and kinetin which was appropriate for the induction and multiplication of callus. Also, this research was conducted in the Biotechnology Laboratory of Agriculture Faculty, Sultan Ageng Tirtayasa University, Banten. From February to June 2018 and used complete random design and repeated three times with two factors and there are two stages, was callus induction and subculture (multiplication). As for some concentrations of growth regulators used in the callus induction stage of 0 ppm (control), 1 ppm, 2 ppm, 3 ppm and 4 ppm for growth regulators 2,4-D and 0 g/l (control), 100 g/l and 150 g/l for tomato extract. While for the subculture stage (multiplication) concentrations of 2,4-D growth regulators used were 1 ppm, 2 ppm, 3 ppm and 4 ppm and concentrations of kinetin growth regulators was 0.5 ppm, 1 ppm and 1,5 ppm. The Research of soursop endosperm culture obtained the best concentration of growth regulators in the application of 2,4-D growth regulators with a concentration of 2 ppm which significantly affected the diameter of soursop endosperm explants at callus induction stage with an average diameter of 5.27 cm. The concentration of 2 ppm and 3 ppm was also estimated for influential the scoring of soursop endosperm explants by the scoring was two. While, the subculture stage (multiplication) the best concentration of growth regulators was 3 ppm of 2,4-D which combined with 1,5 ppm kinetin had an effect on callus formation scoring and the scoring was three. However, for the using of tomato extract, the results were very significant for the explant browning percentage with an average percentage of 0% while for explants grown without tomato extract obtained the highest percentage of 100%.