PENGARUH KONSENTRASI CH3COOH PADA EKSTRAKSI KOLAGEN LIMBAH SISIK IKAN BANDENG DAN PENAMBAHAN AgNO3 TERHADAP NANOFIBER PVA/KOLAGEN UNTUK APLIKASI WOUND DRESSING
| ctrlnum |
15520 |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://eprints.untirta.ac.id/15520/</relation><title>PENGARUH KONSENTRASI CH3COOH PADA EKSTRAKSI KOLAGEN LIMBAH SISIK IKAN BANDENG DAN PENAMBAHAN AgNO3 TERHADAP NANOFIBER PVA/KOLAGEN UNTUK APLIKASI WOUND DRESSING</title><creator>VIRYANINGSIH, NUR</creator><subject>TS Manufactures</subject><description>Fish scale waste can be used as a raw material for producing collagen. Collagen from fish scale waste is widely applied, including one in the medical field, where it is used in the production of wound dressings. However, in previous studies, collagen was not sufficient to be used as an ingredient in the manufacture of wound dressings because it didn't have antibacterial properties that could prevent infection. It is therefore necessary to add materials with antimicrobial properties such as AgNO3. Collagen was created in this study using milkfish scales. This study's procedure involved pretreatment with 0.1 M NaOH solution for 8 hours, neutralization with distilled water to a neutral pH, collagen extraction with CH3COOH solution at concentrations of 0.75 M, 1 M, and 1.25 M for 3x24 hours, salting out with 2.6 M NaCl solution for 24 hours, dialysis, and freeze-drying. The milkfish scale collagen results were then analyzed using yield analysis, water content, ash content, pH, and FTIR to pick the best collagen yield to be utilized as raw material in the following step, which is the manufacturing of nanofibers for wound dressing applications. Based on these results, it is known that the collagen in milkfish scales is obtained using 0.75 M, 1 M, and 1.25 M acetic acid, that is, each obtained a yield value (0.186%; 0.3185%; and 0.2335%), the value of water content (16.67%; 10%; and 3.33%), ash content value (13.33%; 3.33%; and 6.67%), pH value (5.17; 4.61; and 4.29), and FTIR showed amide group A (3292.16 cm-1, 3308.47 cm-1, and 3310.51 cm-1), amide B (2922.96 cm-1, 2927.04 cm-1, and 2925 cm-1), amide I (1639.96 cm-1, 1637.92 cm-1, and 1633.84 cm-1), amide II (1546.13 cm-1, 1544.09 cm-1, and 1542.05 cm-1) and amide III (1238.13 cm-1, 1236.09 cm-1, and 1234.05 cm-1). The milkfish scale collagen used is a concentration of 1 M acetic acid for the manufacture of nanofibers using the electrospinning method consisting of Polyvinyl Alcohol (PVA) based materials, with an electrospinning voltage of 19 kV, a spraying rate of 0,183 ml/hour, a diameter of the electrospinning needle of 0,3 mm, and a distance of the electrospinning needle to the collector of 15 cm. The treatment was then completed with 0.1 M AgNO3 and without AgNO3. The nanofiber's results were examined for X-Ray Diffraction (XRD) and antibacterial activity. Based on these results, it was discovered that the Ag compound peaks on PVA/Collagen/AgNO3 (45,35° and 51,74°) nanofibers, peaks of collagen compounds on PVA/Collagen nanofibers and PVA/Collagen/AgNO3 nanofibers (23,11° and 22,25°), peaks of PVA compounds found in PVA/Collagen nanofibers (22,01°; 43,73° and 64,12°), and PVA/Collagen/AgNO3 nanofibers at 2θ angles were (21,41° and 64,50°). Adding AgNO3 to PVA/collagen nanofibers produced an inhibition zone of 1 mm when antibacterial compounds were tested for inhibition. In addition, examining the inhibition of antibacterial compounds produced by adding AgNO3 to PVA/collagen nanofibers resulted in a 1 mm inhibition zone.</description><date>2022-09-06</date><type>Thesis:Thesis</type><type>PeerReview:NonPeerReviewed</type><type>Book:Book</type><language>ind</language><identifier>https://eprints.untirta.ac.id/15520/1/3334170015.pdf</identifier><type>Book:Book</type><language>ind</language><identifier>https://eprints.untirta.ac.id/15520/2/Nur%20Viryaningsih_3334170015_01.pdf</identifier><type>Book:Book</type><language>ind</language><identifier>https://eprints.untirta.ac.id/15520/3/Nur%20Viryaningsih_3334170015_02.pdf</identifier><type>Book:Book</type><language>ind</language><identifier>https://eprints.untirta.ac.id/15520/4/Nur%20Viryaningsih_3334170015_03.pdf</identifier><type>Book:Book</type><language>ind</language><identifier>https://eprints.untirta.ac.id/15520/5/Nur%20Viryaningsih_3334170015_04.pdf</identifier><type>Book:Book</type><language>ind</language><identifier>https://eprints.untirta.ac.id/15520/6/Nur%20Viryaningsih_3334170015_05.pdf</identifier><type>Book:Book</type><language>ind</language><identifier>https://eprints.untirta.ac.id/15520/7/Nur%20Viryaningsih_3334170015_Ref.pdf</identifier><type>Book:Book</type><language>ind</language><identifier>https://eprints.untirta.ac.id/15520/8/Nur%20Viryaningsih_3334170015_Lamp.pdf</identifier><identifier> VIRYANINGSIH, NUR (2022) PENGARUH KONSENTRASI CH3COOH PADA EKSTRAKSI KOLAGEN LIMBAH SISIK IKAN BANDENG DAN PENAMBAHAN AgNO3 TERHADAP NANOFIBER PVA/KOLAGEN UNTUK APLIKASI WOUND DRESSING. S1 thesis, UNIVERSITAS SULTAN AGENG TIRTAYASA. </identifier><recordID>15520</recordID></dc>
|
| language |
ind |
| format |
Thesis:Thesis Thesis PeerReview:NonPeerReviewed PeerReview Book:Book Book |
| author |
VIRYANINGSIH, NUR |
| title |
PENGARUH KONSENTRASI CH3COOH PADA EKSTRAKSI KOLAGEN LIMBAH SISIK IKAN BANDENG DAN PENAMBAHAN AgNO3 TERHADAP NANOFIBER PVA/KOLAGEN UNTUK APLIKASI WOUND DRESSING |
| publishDate |
2022 |
| isbn |
9783334170014 |
| topic |
TS Manufactures |
| url |
https://eprints.untirta.ac.id/15520/1/3334170015.pdf https://eprints.untirta.ac.id/15520/2/Nur%20Viryaningsih_3334170015_01.pdf https://eprints.untirta.ac.id/15520/3/Nur%20Viryaningsih_3334170015_02.pdf https://eprints.untirta.ac.id/15520/4/Nur%20Viryaningsih_3334170015_03.pdf https://eprints.untirta.ac.id/15520/5/Nur%20Viryaningsih_3334170015_04.pdf https://eprints.untirta.ac.id/15520/6/Nur%20Viryaningsih_3334170015_05.pdf https://eprints.untirta.ac.id/15520/7/Nur%20Viryaningsih_3334170015_Ref.pdf https://eprints.untirta.ac.id/15520/8/Nur%20Viryaningsih_3334170015_Lamp.pdf https://eprints.untirta.ac.id/15520/ |
| contents |
Fish scale waste can be used as a raw material for producing collagen. Collagen from fish scale waste is widely applied, including one in the medical field, where it is used in the production of wound dressings. However, in previous studies, collagen was not sufficient to be used as an ingredient in the manufacture of wound dressings because it didn't have antibacterial properties that could prevent infection. It is therefore necessary to add materials with antimicrobial properties such as AgNO3. Collagen was created in this study using milkfish scales. This study's procedure involved pretreatment with 0.1 M NaOH solution for 8 hours, neutralization with distilled water to a neutral pH, collagen extraction with CH3COOH solution at concentrations of 0.75 M, 1 M, and 1.25 M for 3x24 hours, salting out with 2.6 M NaCl solution for 24 hours, dialysis, and freeze-drying. The milkfish scale collagen results were then analyzed using yield analysis, water content, ash content, pH, and FTIR to pick the best collagen yield to be utilized as raw material in the following step, which is the manufacturing of nanofibers for wound dressing applications. Based on these results, it is known that the collagen in milkfish scales is obtained using 0.75 M, 1 M, and 1.25 M acetic acid, that is, each obtained a yield value (0.186%; 0.3185%; and 0.2335%), the value of water content (16.67%; 10%; and 3.33%), ash content value (13.33%; 3.33%; and 6.67%), pH value (5.17; 4.61; and 4.29), and FTIR showed amide group A (3292.16 cm-1, 3308.47 cm-1, and 3310.51 cm-1), amide B (2922.96 cm-1, 2927.04 cm-1, and 2925 cm-1), amide I (1639.96 cm-1, 1637.92 cm-1, and 1633.84 cm-1), amide II (1546.13 cm-1, 1544.09 cm-1, and 1542.05 cm-1) and amide III (1238.13 cm-1, 1236.09 cm-1, and 1234.05 cm-1). The milkfish scale collagen used is a concentration of 1 M acetic acid for the manufacture of nanofibers using the electrospinning method consisting of Polyvinyl Alcohol (PVA) based materials, with an electrospinning voltage of 19 kV, a spraying rate of 0,183 ml/hour, a diameter of the electrospinning needle of 0,3 mm, and a distance of the electrospinning needle to the collector of 15 cm. The treatment was then completed with 0.1 M AgNO3 and without AgNO3. The nanofiber's results were examined for X-Ray Diffraction (XRD) and antibacterial activity. Based on these results, it was discovered that the Ag compound peaks on PVA/Collagen/AgNO3 (45,35° and 51,74°) nanofibers, peaks of collagen compounds on PVA/Collagen nanofibers and PVA/Collagen/AgNO3 nanofibers (23,11° and 22,25°), peaks of PVA compounds found in PVA/Collagen nanofibers (22,01°; 43,73° and 64,12°), and PVA/Collagen/AgNO3 nanofibers at 2θ angles were (21,41° and 64,50°). Adding AgNO3 to PVA/collagen nanofibers produced an inhibition zone of 1 mm when antibacterial compounds were tested for inhibition. In addition, examining the inhibition of antibacterial compounds produced by adding AgNO3 to PVA/collagen nanofibers resulted in a 1 mm inhibition zone. |
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IOS3442.15520 |
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Universitas Sultan Ageng Tirtayasa |
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89 |
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library:university library |
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Perpustakaan Universitas Sultan Ageng Tirtayasa |
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598 |
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Repository Universitas Sultan Ageng Tirtayasa |
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3442 |
| subject_area |
Administrasi Negara dan Militer Akuntansi Arsitektur |
| city |
KOTA SERANG |
| province |
BANTEN |
| repoId |
IOS3442 |
| first_indexed |
2023-03-04T21:43:48Z |
| last_indexed |
2023-04-29T09:38:59Z |
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dc |
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1764503586435235840 |
| score |
17.538404 |