The Influence of Magnetic Fields Supply and Hygromycin Concentration to the Optimization of Folate Gene Putative Transformant Selection in Rice (cv. Fatmawati)
| Main Authors: | Susiyanti, Susiyanti, Nurmayulis, Nurmayulis, Sjaifuddin, Sjaifuddin, WATI, WINDA, Isminingsih, Sulastri |
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| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Atlantis Press
, 2021
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| Subjects: | |
| Online Access: |
https://eprints.untirta.ac.id/11018/1/16.%20din_atlantis_magneticfields.pdf https://eprints.untirta.ac.id/11018/ https://www.atlantis-press.com/proceedings/icfsi-19/125953491 https://doi.org/10.2991/absr.k.210304.046 |
Daftar Isi:
- The research was aimed to determine the influence of magnetic fields supply and hygromycin concentration on the optimization of folate gene putative transformant selection in rice (cv. Fatmawati). The Genetic transformation of rice used A. tumefaciens strain LBA4404 as a vector that contained plasmid pCambia1300-CGH1 construct through in planta transformation method using scutellum tissue explant. This research was conducted at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Sultan Ageng Tirtayasa University, Banten (February to April 2019). This research used a completely randomized design consisting of two factors. The first factor was the supply of a magnetic field, with two levels: without magnet and magnet. The second was the hygromycin concentrations with 4 levels: 25, 50, 75, and 100 mg.L-1 The results of the magnetic fields did not affect the transformation efficiency parameters and the percentage of explant deaths. The results of different hygromycin concentrations showed an effect on transformation efficiency parameters and percentage of explants deaths. The concentration of hygromycin 25 mg.L-1 had the highest average of transformation efficiency 100% based on the number of explants who passed the selection media suspected of being putative transformants and the low percentage of explants deaths 0%. In the lethal test of concentration-50 (LC50) hygromycin, the concentration of hygromycin 75 mg.L-1 caused up to 50% mortality in rice cv. Fatmawati explants. There had no interaction between the magnetic field supply and the hygromycin concentration on the parameters of transformation efficiency and the percentage of explant deaths.