STUDI ON GENETIC DIVERSITY OF Diospyros rumphii Bakh IN NORTH SULAWESI BASED ON ISOENZYM MARKERS
| Main Authors: | Kinho, Julianus, Na'iem, Mohammad, Indrioko, Sapto |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Center for Forest Biotechnology and Tree Improvement (CFBTI)
, 2016
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| Subjects: | |
| Online Access: |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/2397 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/2397/1882 |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">STUDI ON GENETIC DIVERSITY OF Diospyros rumphii Bakh IN NORTH SULAWESI BASED ON ISOENZYM MARKERS</title><creator>Kinho, Julianus</creator><creator>Na'iem, Mohammad</creator><creator>Indrioko, Sapto</creator><subject lang="en-US">Diospyros rumphii; isoenzym; population; genetic diversity</subject><description lang="en-US">Diospyros rumphii Bakh., is one of tropical commercial wood species grouped in ebony. Ebony is the name of some species of commercial timber from genus of Diospyros (Ebenaceae). Seven species of them are  commercial wood, which in the trade is known as ebony namely; D. celebica, D. rumphii, D. pilosanthera, D. lolin, D. ebenum, D. ferrea and D. macrophylla. Two of seven species ebony have the highest economic values namely; D. celebica and D. rumphii. Nowadays, the population D. rumphii in the nature are decreases due to over harvesting in the past. This study aims to determine genetic diversity of D. rumphii within populations, between populations and its relationship. The samples of D. rumphii in this study from four populations in North Sulawesi i.e Bitung, Talise, Talaud and Bolaang Mongondow. Genetic diversity was detected by isoenzym markers with seven enzyme i.e Glutamate oxaloacetate transmirase (GOT), Shikimate dehydrogenase (SHD), Esterase (EST), Acid phoshatase (ACP), Diaphorase (DIA), 6-Phosphogluconate dehydrogenase (6PG) and Glutamate dehydrogenase (GDH). The results showed that there are three enzym was detected   polymorphisme EST, GOT and GDH. Genetic diversity generated by three enzyme systems who are controlled by 5 loci and 15 alleles. The average percentage of polymorphic loci was 100%. The number of total genetic diversity is 0.64, which are distributed within population 0.59 (91.24%) and between population 0.05 (8.76%). Cluster analysis using Unweighted Pair Group Method with Aritmatic Means (UPGMA) based on Nei standard genetic distance showed three clusters. The first clusters are population of Bitung and Bolaang Mongondow, the second cluster is population of Talise, and the third cluster is population of Talaud who are separate far from the other populations.</description><publisher lang="en-US">Center for Forest Biotechnology and Tree Improvement (CFBTI)</publisher><contributor lang="en-US"/><date>2016-12-15</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/2397</identifier><identifier>10.20886/jpth.2016.10.2.95-109</identifier><source lang="en-US">Jurnal Pemuliaan Tanaman Hutan; Vol 10, No 2 (2016): Jurnal Pemuliaan Tanaman Hutan; 95-109</source><source>2527-8665</source><source>1693-7147</source><language>eng</language><relation>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/2397/1882</relation><rights lang="en-US">Copyright (c) 2016 Julianus Kinho, Mohammad Na'iem, Sapto Indrioko</rights><rights lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</rights><recordID>--ejournal.forda-mof.org-ejournal-litbang-index.php-index-oai:article-2397</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other Other: File:application/pdf File Journal:eJournal |
| author |
Kinho, Julianus Na'iem, Mohammad Indrioko, Sapto |
| title |
STUDI ON GENETIC DIVERSITY OF Diospyros rumphii Bakh IN NORTH SULAWESI BASED ON ISOENZYM MARKERS |
| publisher |
Center for Forest Biotechnology and Tree Improvement (CFBTI) |
| publishDate |
2016 |
| topic |
Diospyros rumphii isoenzym population genetic diversity |
| url |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/2397 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/2397/1882 |
| contents |
Diospyros rumphii Bakh., is one of tropical commercial wood species grouped in ebony. Ebony is the name of some species of commercial timber from genus of Diospyros (Ebenaceae). Seven species of them are commercial wood, which in the trade is known as ebony namely; D. celebica, D. rumphii, D. pilosanthera, D. lolin, D. ebenum, D. ferrea and D. macrophylla. Two of seven species ebony have the highest economic values namely; D. celebica and D. rumphii. Nowadays, the population D. rumphii in the nature are decreases due to over harvesting in the past. This study aims to determine genetic diversity of D. rumphii within populations, between populations and its relationship. The samples of D. rumphii in this study from four populations in North Sulawesi i.e Bitung, Talise, Talaud and Bolaang Mongondow. Genetic diversity was detected by isoenzym markers with seven enzyme i.e Glutamate oxaloacetate transmirase (GOT), Shikimate dehydrogenase (SHD), Esterase (EST), Acid phoshatase (ACP), Diaphorase (DIA), 6-Phosphogluconate dehydrogenase (6PG) and Glutamate dehydrogenase (GDH). The results showed that there are three enzym was detected polymorphisme EST, GOT and GDH. Genetic diversity generated by three enzyme systems who are controlled by 5 loci and 15 alleles. The average percentage of polymorphic loci was 100%. The number of total genetic diversity is 0.64, which are distributed within population 0.59 (91.24%) and between population 0.05 (8.76%). Cluster analysis using Unweighted Pair Group Method with Aritmatic Means (UPGMA) based on Nei standard genetic distance showed three clusters. The first clusters are population of Bitung and Bolaang Mongondow, the second cluster is population of Talise, and the third cluster is population of Talaud who are separate far from the other populations. |
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