KERAGAMAN GENETIK POPULASI Araucaria cunninghamii MENGGUNAKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA)

Main Authors: Widyatmoko, AYPBC, Patrisia Lejo, Elisabeth Selda, Prasetyaningsih, Aniek, Rimbawanto, Anto
Format: Article info eJournal
Bahasa: eng
Terbitan: BBPPBPTH , 2010
Subjects:
Online Access: http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1838
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1838/1613
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fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">KERAGAMAN GENETIK POPULASI Araucaria cunninghamii MENGGUNAKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA)</title><creator>Widyatmoko, AYPBC</creator><creator>Patrisia Lejo, Elisabeth Selda</creator><creator>Prasetyaningsih, Aniek</creator><creator>Rimbawanto, Anto</creator><subject lang="en-US">Araucaria cunninghamii, genetic diversity, RAPD</subject><description lang="en-US">Genetic diversity was needed for supporting genetic conservation and tree improvement program. Araucaria&#xA0; cunnighamii is one of Indonesian conifer species which has various potential uses. Information about genetic diversity and its distribution, and genetic relationship can be revealed using RAPD (Random Amplified Polymorphic DNA) molecular marker. The objective of the study was to analyze genetic diversity within and among 8 populations of A. Cunninghamii, and genetic relationship between populations. Leaf samples of 64 trees were collected from 7 population in Papua and 1 population in Queensland (Australia). Genetic diversity was analyzed using 23 RAPD primers with 68 polymorphic loci. Mean genetic diversity within population was 0.270, and mean genetic distance among populations was 0.092. Some of the samples from the same population were grouped in the same cluster. But generally, the clustering of the samples. Genetic relationship among A. cunninghamii populations revealed that 8 populations were divided into two groups. First group was consisted of 7 population in Papua (Indonesia) and then split into 5 subcluster. Second group was population in Queensland (Australia).</description><publisher lang="en-US">BBPPBPTH</publisher><contributor lang="en-US"/><date>2010-09-15</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>Other:</type><identifier>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1838</identifier><identifier>10.20886/jpth.2015.9.3.131-202</identifier><source lang="en-US">Jurnal Pemuliaan Tanaman Hutan; Vol 4, No 2 (2010): Jurnal Pemuliaan Tanaman Hutan; 63-77</source><source>2527-8665</source><source>1693-7147</source><language>eng</language><relation>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1838/1613</relation><rights lang="en-US">Copyright (c) 2016 Jurnal Pemuliaan Tanaman Hutan</rights><rights lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</rights><recordID>--ejournal.forda-mof.org-ejournal-litbang-index.php-index-oai:article-1838</recordID></dc>
language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
Other:
Journal:eJournal
author Widyatmoko, AYPBC
Patrisia Lejo, Elisabeth Selda
Prasetyaningsih, Aniek
Rimbawanto, Anto
title KERAGAMAN GENETIK POPULASI Araucaria cunninghamii MENGGUNAKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA)
publisher BBPPBPTH
publishDate 2010
topic Araucaria cunninghamii
genetic diversity
RAPD
url http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1838
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1838/1613
contents Genetic diversity was needed for supporting genetic conservation and tree improvement program. Araucaria cunnighamii is one of Indonesian conifer species which has various potential uses. Information about genetic diversity and its distribution, and genetic relationship can be revealed using RAPD (Random Amplified Polymorphic DNA) molecular marker. The objective of the study was to analyze genetic diversity within and among 8 populations of A. Cunninghamii, and genetic relationship between populations. Leaf samples of 64 trees were collected from 7 population in Papua and 1 population in Queensland (Australia). Genetic diversity was analyzed using 23 RAPD primers with 68 polymorphic loci. Mean genetic diversity within population was 0.270, and mean genetic distance among populations was 0.092. Some of the samples from the same population were grouped in the same cluster. But generally, the clustering of the samples. Genetic relationship among A. cunninghamii populations revealed that 8 populations were divided into two groups. First group was consisted of 7 population in Papua (Indonesia) and then split into 5 subcluster. Second group was population in Queensland (Australia).
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