Variasi Genetik Pinus merkusii Menggunakan Penanda Mikrosatelit Kloroplas
| Main Authors: | Nurtjahjaningsih, ILG, Widyatmoko, AYPBC, Rimbawanto, Anto |
|---|---|
| Format: | Article info eJournal |
| Bahasa: | eng |
| Terbitan: |
BBPPBPTH
, 2011
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| Subjects: | |
| Online Access: |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1823 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1823/1598 |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Variasi Genetik Pinus merkusii Menggunakan Penanda Mikrosatelit Kloroplas</title><creator>Nurtjahjaningsih, ILG</creator><creator>Widyatmoko, AYPBC</creator><creator>Rimbawanto, Anto</creator><subject lang="en-US">Pinus merkusii; genetic variation;chloroplast microsatelite</subject><description lang="en-US">Using two chloroplast universal primers, haplotipic within and among populations of Pinus merkusii had been evaluated, in order to access paternal variation of this species. The aim of this study was to investigate variation of chloroplast microsatellite in P. merkusii. Using two universal chloroplast microsatelites, a total of 18 chloroplast haplotypes were found among 730 individuals surveyed in 10populations in Java. Private haplotypes mostly found at eastern Java populations. The number ofdetected haplotypes (Na) within populations ranged between 5 and 14. Variation of haplotypes (h) was relatively hight; it ranged between 0.727 and 0.858. Nei’s genetic distance among populations was very low (Da=0.030). Moreover, analysis of molecular variance (AMOVA) showed genetic variation originated from within population but the value was insignificant.It was concluded that private haplotypes found in eastern Java might show gene pool of P. merkusii in Java. These detected haplotypes are of important information for genetic resources conservation anf breeding strategy of P. merkusii.</description><publisher lang="en-US">BBPPBPTH</publisher><contributor lang="en-US"/><date>2011-11-15</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>Other:</type><identifier>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1823</identifier><identifier>10.20886/jpth.2015.9.3.131-202</identifier><source lang="en-US">Jurnal Pemuliaan Tanaman Hutan; Vol 5, No 3 (2011): Jurnal Pemuliaan Tanaman Hutan; 119-128</source><source>2527-8665</source><source>1693-7147</source><language>eng</language><relation>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1823/1598</relation><rights lang="en-US">Copyright (c) 2016 Jurnal Pemuliaan Tanaman Hutan</rights><rights lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</rights><recordID>--ejournal.forda-mof.org-ejournal-litbang-index.php-index-oai:article-1823</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other Other: Journal:eJournal |
| author |
Nurtjahjaningsih, ILG Widyatmoko, AYPBC Rimbawanto, Anto |
| title |
Variasi Genetik Pinus merkusii Menggunakan Penanda Mikrosatelit Kloroplas |
| publisher |
BBPPBPTH |
| publishDate |
2011 |
| topic |
Pinus merkusii genetic variation chloroplast microsatelite |
| url |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1823 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1823/1598 |
| contents |
Using two chloroplast universal primers, haplotipic within and among populations of Pinus merkusii had been evaluated, in order to access paternal variation of this species. The aim of this study was to investigate variation of chloroplast microsatellite in P. merkusii. Using two universal chloroplast microsatelites, a total of 18 chloroplast haplotypes were found among 730 individuals surveyed in 10populations in Java. Private haplotypes mostly found at eastern Java populations. The number ofdetected haplotypes (Na) within populations ranged between 5 and 14. Variation of haplotypes (h) was relatively hight; it ranged between 0.727 and 0.858. Nei’s genetic distance among populations was very low (Da=0.030). Moreover, analysis of molecular variance (AMOVA) showed genetic variation originated from within population but the value was insignificant.It was concluded that private haplotypes found in eastern Java might show gene pool of P. merkusii in Java. These detected haplotypes are of important information for genetic resources conservation anf breeding strategy of P. merkusii. |
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Kementerian Lingkungan Hidup dan Kehutanan Republik Indonesia |
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