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SCREENING PENANDA MIKROSATELIT Shorea curtisii TERHADAP JENIS-JENIS SHOREA PENGHASIL TENGKAWANG

Main Authors: Nurtjahjaningsih, ILG, Widyatmoko, AYPBC, Sulistyawati, Purnamila, Rimbawanto, Anto
Format: Article info eJournal
Bahasa: eng
Terbitan: BBPPBPTH , 2012
Subjects:
screening microsatellite
genus Shorea
shared allele
expected heterozygosity
coefficient of inbreeding
Online Access: http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1686
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1686/1505
ctrlnum --ejournal.forda-mof.org-ejournal-litbang-index.php-index-oai:article-1686
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">SCREENING PENANDA MIKROSATELIT Shorea curtisii TERHADAP JENIS-JENIS SHOREA PENGHASIL TENGKAWANG</title><creator>Nurtjahjaningsih, ILG</creator><creator>Widyatmoko, AYPBC</creator><creator>Sulistyawati, Purnamila</creator><creator>Rimbawanto, Anto</creator><subject lang="en-US">screening microsatellite; genus Shorea; shared allele; expected heterozygosity; coefficient of inbreeding</subject><description lang="en-US">Screening primer is an effective method to develop microsatellite markers from related taxa. Aim of this study&#xA0;was to develop microsatellite markers of four Shorea producing tengkawang oil, i.e. Shorea gysbertiana, Shorea macrophylla, Shorea pinanga and Shorea stenoptera by screening microsatellite primers of Shorea curtisii. Leaf samples of the four Shorea were collected from nursery at Center for Forest Biotechnology and Tree Improvement Research in Yogyakarta. Four microsatellite primers of S. curtisii i.e. Shc-1, Shc-2, Shc-7 and Shc-9 had been used to screen. Results showed that numerous alleles were shared among the Shorea. The expected heterozygosity (HE) for locus Shc-1 ranged between 0.594 and 0.722; locus Shc-2 ranged between 0.219 and 0.611; locus Shc-7 ranged between 0.594 and 0.778; and locus Shc-9 ranged between 0.594 and 0.844. Coefficient of inbreeding (FIS) value was low and it was insignificant deviated from Hardy-Weinberg Equilibrium (HWE) at&#xA0; almost all loci except Shc-1 of S.&#xA0; pinanga. A dendrogram showed two clusters; S. gysbertsiana and S. macrophylla represented in one cluster, while S. pinanga and S. stenoptera represented in another cluster. Therefore the developed microsatellite markers are possible&#xA0;to be applied for studying population genetics and mating system of these species.</description><publisher lang="en-US">BBPPBPTH</publisher><contributor lang="en-US"/><date>2012-07-15</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>Other:</type><identifier>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1686</identifier><identifier>10.20886/jpth</identifier><source lang="en-US">Jurnal Pemuliaan Tanaman Hutan; Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan; 49-56</source><source>2527-8665</source><source>1693-7147</source><language>eng</language><relation>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1686/1505</relation><rights lang="en-US">Copyright (c) 2016 Jurnal Pemuliaan Tanaman Hutan</rights><rights lang="en-US">http://creativecommons.org/licenses/by-nc/4.0</rights><recordID>--ejournal.forda-mof.org-ejournal-litbang-index.php-index-oai:article-1686</recordID></dc>
language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
Other:
Journal:eJournal
author Nurtjahjaningsih, ILG
Widyatmoko, AYPBC
Sulistyawati, Purnamila
Rimbawanto, Anto
title SCREENING PENANDA MIKROSATELIT Shorea curtisii TERHADAP JENIS-JENIS SHOREA PENGHASIL TENGKAWANG
publisher BBPPBPTH
publishDate 2012
topic screening microsatellite
genus Shorea
shared allele
expected heterozygosity
coefficient of inbreeding
url http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1686
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/1686/1505
contents Screening primer is an effective method to develop microsatellite markers from related taxa. Aim of this study was to develop microsatellite markers of four Shorea producing tengkawang oil, i.e. Shorea gysbertiana, Shorea macrophylla, Shorea pinanga and Shorea stenoptera by screening microsatellite primers of Shorea curtisii. Leaf samples of the four Shorea were collected from nursery at Center for Forest Biotechnology and Tree Improvement Research in Yogyakarta. Four microsatellite primers of S. curtisii i.e. Shc-1, Shc-2, Shc-7 and Shc-9 had been used to screen. Results showed that numerous alleles were shared among the Shorea. The expected heterozygosity (HE) for locus Shc-1 ranged between 0.594 and 0.722; locus Shc-2 ranged between 0.219 and 0.611; locus Shc-7 ranged between 0.594 and 0.778; and locus Shc-9 ranged between 0.594 and 0.844. Coefficient of inbreeding (FIS) value was low and it was insignificant deviated from Hardy-Weinberg Equilibrium (HWE) at almost all loci except Shc-1 of S. pinanga. A dendrogram showed two clusters; S. gysbertsiana and S. macrophylla represented in one cluster, while S. pinanga and S. stenoptera represented in another cluster. Therefore the developed microsatellite markers are possible to be applied for studying population genetics and mating system of these species.
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