STRUKTUR GENETIK POHON INDUK Calophyllum inophyllum DI TEGAKAN BENIH PROVENAN BERDASARKAN PENANDA SIMPLE SEQUENCES REPEATS
| Main Authors: | Nurtjahjaningsih, ILG, Sulistyawati, Purnamila, Rimbawanto, Anto |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Center for Forest Biotechnology and Tree Improvement (CFBTI)
, 2019
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| Subjects: | |
| Online Access: |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5272 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5272/4743 |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">STRUKTUR GENETIK POHON INDUK Calophyllum inophyllum DI TEGAKAN BENIH PROVENAN BERDASARKAN PENANDA SIMPLE SEQUENCES REPEATS</title><creator>Nurtjahjaningsih, ILG</creator><creator>Sulistyawati, Purnamila</creator><creator>Rimbawanto, Anto</creator><subject lang="id-ID">gene diversity; genetic differentiation; inbreeding</subject><description lang="id-ID">This study aimed to assess genetic structure of Calophyllum inophyllum using simple sequences repeats (SSR) markers to control genetic diversity at a provenance seed stand. Leaf samples were collected for DNA templates and 6 SSR primers were used. Genetic data of 280 trees were analyzed by FSTAT software, while PCoA was analyzed by GeneAlex. Results showed that number of allele (NA) per SSR primer between 3 and 4, the allelic richness (AR) ranged 2.805 to 4.000, the gene diversity (HE) ranged 0.018 to 0.631, coefficient inbreeding (FIS) of overall SSR primers were insignificant deviate from Hardy-Weinberg Equilibrium, excepted NY3. The mean HE values in the TBP was in low (mean HE= 0.272). However, the mean coefficient inbreeding values was not significant. PCoA analysis showed that the seed stand consisted genetically related trees; the 280 trees originated from only 5-6 mother trees. This is because the stand was originated from a conservation stand with narrow genetic differentiation. Management of the seed stand was discussed.</description><publisher lang="en-US">Center for Forest Biotechnology and Tree Improvement (CFBTI)</publisher><contributor lang="id-ID"/><date>2019-06-30</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5272</identifier><identifier>10.20886/jpth.2019.13.1.45-51</identifier><source lang="en-US">Jurnal Pemuliaan Tanaman Hutan; Vol 13, No 1 (2019): Jurnal Pemuliaan Tanaman Hutan; 45-51</source><source>2527-8665</source><source>1693-7147</source><language>eng</language><relation>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5272/4743</relation><rights lang="en-US">Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan</rights><rights lang="en-US">https://creativecommons.org/licenses/by-nc-sa/4.0</rights><recordID>--ejournal.forda-mof.org-ejournal-litbang-index.php-index-oai?verb=ListSets:article-5272</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other Other: File:application/pdf File Journal:eJournal |
| author |
Nurtjahjaningsih, ILG Sulistyawati, Purnamila Rimbawanto, Anto |
| title |
STRUKTUR GENETIK POHON INDUK Calophyllum inophyllum DI TEGAKAN BENIH PROVENAN BERDASARKAN PENANDA SIMPLE SEQUENCES REPEATS |
| publisher |
Center for Forest Biotechnology and Tree Improvement (CFBTI) |
| publishDate |
2019 |
| topic |
gene diversity genetic differentiation inbreeding |
| url |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5272 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5272/4743 |
| contents |
This study aimed to assess genetic structure of Calophyllum inophyllum using simple sequences repeats (SSR) markers to control genetic diversity at a provenance seed stand. Leaf samples were collected for DNA templates and 6 SSR primers were used. Genetic data of 280 trees were analyzed by FSTAT software, while PCoA was analyzed by GeneAlex. Results showed that number of allele (NA) per SSR primer between 3 and 4, the allelic richness (AR) ranged 2.805 to 4.000, the gene diversity (HE) ranged 0.018 to 0.631, coefficient inbreeding (FIS) of overall SSR primers were insignificant deviate from Hardy-Weinberg Equilibrium, excepted NY3. The mean HE values in the TBP was in low (mean HE= 0.272). However, the mean coefficient inbreeding values was not significant. PCoA analysis showed that the seed stand consisted genetically related trees; the 280 trees originated from only 5-6 mother trees. This is because the stand was originated from a conservation stand with narrow genetic differentiation. Management of the seed stand was discussed. |
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Kementerian Lingkungan Hidup dan Kehutanan Republik Indonesia |
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