PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis)
| Main Authors: | Nurtjahjaningsih, ILG, Herawan, Toni, Rachma, Reza Permatasari, Rimbawanto, Anto |
|---|---|
| Other Authors: | BBPPBPTH |
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Center for Forest Biotechnology and Tree Improvement (CFBTI)
, 2018
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| Subjects: | |
| Online Access: |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4758 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4758/4618 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/downloadSuppFile/4758/450 |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis)</title><creator>Nurtjahjaningsih, ILG</creator><creator>Herawan, Toni</creator><creator>Rachma, Reza Permatasari</creator><creator>Rimbawanto, Anto</creator><subject lang="id-ID">Tissue culture; primers screening; polymorphic loci; somaclonal variation</subject><description lang="id-ID">This study aimed to test RAPD markers to assess genetic stability of teak clones. Two experimental steps were carried out. First, nine RAPD markers were screened to verify the level of polymorphic loci; second, the polymorphic loci were applied to test genetic stability of clones. To test polymorphism levels of the primers, DNA was isolated from eight leaf samples that were collected from a seed orchard located at Watusipat, Gunung Kidul. To verify genetic stability of clones, DNA was isolated from leaf samples of 24 ramets of 3 clones after second sub-culturing. Results showed that amplification of 5 out of 9 RAPD primers were be consisten and produced 12 polymorphic loci. The number of polymorphic alleles per locus ranged between 1 and 3; the allele sizes were between 400 and 1,050 base pairs (bps). The percentage of polymorphic loci was 100%; it meant that overall loci have high polymorphism level. Based on these loci showed that the 24 ramets are clones; there was no somaclonal variation or high genetic stability. However, these loci need to be validated using more stable DNA markers.</description><publisher lang="en-US">Center for Forest Biotechnology and Tree Improvement (CFBTI)</publisher><contributor lang="id-ID">BBPPBPTH</contributor><date>2018-12-31</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4758</identifier><identifier>10.20886/jpth.2018.12.2.127-134</identifier><source lang="en-US">Jurnal Pemuliaan Tanaman Hutan; Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan; 127-134</source><source>2527-8665</source><source>1693-7147</source><language>eng</language><relation>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4758/4618</relation><relation>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/downloadSuppFile/4758/450</relation><rights lang="en-US">Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan</rights><rights lang="en-US">https://creativecommons.org/licenses/by-nc-sa/4.0</rights><recordID>--ejournal.forda-mof.org-ejournal-litbang-index.php-index-oai?verb=ListSets:article-4758</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other Other: File:application/pdf File Journal:eJournal |
| author |
Nurtjahjaningsih, ILG Herawan, Toni Rachma, Reza Permatasari Rimbawanto, Anto |
| author2 |
BBPPBPTH |
| title |
PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis) |
| publisher |
Center for Forest Biotechnology and Tree Improvement (CFBTI) |
| publishDate |
2018 |
| topic |
Tissue culture primers screening polymorphic loci somaclonal variation |
| url |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4758 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4758/4618 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/downloadSuppFile/4758/450 |
| contents |
This study aimed to test RAPD markers to assess genetic stability of teak clones. Two experimental steps were carried out. First, nine RAPD markers were screened to verify the level of polymorphic loci; second, the polymorphic loci were applied to test genetic stability of clones. To test polymorphism levels of the primers, DNA was isolated from eight leaf samples that were collected from a seed orchard located at Watusipat, Gunung Kidul. To verify genetic stability of clones, DNA was isolated from leaf samples of 24 ramets of 3 clones after second sub-culturing. Results showed that amplification of 5 out of 9 RAPD primers were be consisten and produced 12 polymorphic loci. The number of polymorphic alleles per locus ranged between 1 and 3; the allele sizes were between 400 and 1,050 base pairs (bps). The percentage of polymorphic loci was 100%; it meant that overall loci have high polymorphism level. Based on these loci showed that the 24 ramets are clones; there was no somaclonal variation or high genetic stability. However, these loci need to be validated using more stable DNA markers. |
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Perpustakaan Kementerian Lingkungan Hidup dan Kehutanan RI |
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Indonesian Journal of Forestry Research |
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