PENGUJIAN PENANDA JENIS SPESIFIK PADA JAMUR YANG BERPOTENSI SEBAGAI AGENS PENGENDALI HAYATI PENYAKIT BUSUK AKAR PADA AKASIA
| Main Authors: | Prihatini, Istiana, Rimbawanto, Anto, Puspitasari, Desy, Fauzi, Dayin |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Center for Forest Biotechnology and Tree Improvement (CFBTI)
, 2018
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| Subjects: | |
| Online Access: |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4276 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4276/4253 |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">PENGUJIAN PENANDA JENIS SPESIFIK PADA JAMUR YANG BERPOTENSI SEBAGAI AGENS PENGENDALI HAYATI PENYAKIT BUSUK AKAR PADA AKASIA</title><creator>Prihatini, Istiana</creator><creator>Rimbawanto, Anto</creator><creator>Puspitasari, Desy</creator><creator>Fauzi, Dayin</creator><subject lang="id-ID">DNA dilution; Ganoderma; PCR primers; rapid identification</subject><description lang="id-ID">Root rot caused by Ganoderma philippii (Bres. & Henn. ex Sacc.) Bres., is an important disease in Acacia plantation. A strategy to control this disease is currently being developed, particularly on the application of biological control agent (BCA). Species specific primers for rapid identification of potential fungi as BCA were developed. This study aimed to obtain the best DNA condition and the best primers for species specific identification. DNA with 20x dilution is the best condition for amplification of the ITS fragment thus used for rapid species identification. The best primer set to detect Cerrena sp. is CrF1/CrR1, PbF2/PbR2 is the best primer set for Phlebiopsis sp. 1. There was no specific primer suitable to detect Phlebia sp. 1 and Phlebia sp. 2 only but Pl-2F1/Pl-2R4 is the best primer set for Phlebia spp.</description><publisher lang="en-US">Center for Forest Biotechnology and Tree Improvement (CFBTI)</publisher><contributor lang="id-ID"/><date>2018-06-08</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4276</identifier><identifier>10.20886/jpth.2018.12.1.1-11</identifier><source lang="en-US">Jurnal Pemuliaan Tanaman Hutan; Vol 12, No 1 (2018): Jurnal Pemuliaan Tanaman Hutan; 1-11</source><source>2527-8665</source><source>1693-7147</source><language>eng</language><relation>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4276/4253</relation><rights lang="en-US">Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan</rights><rights lang="en-US">https://creativecommons.org/licenses/by-nc-sa/4.0</rights><recordID>--ejournal.forda-mof.org-ejournal-litbang-index.php-index-oai?verb=ListSets:article-4276</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other Other: File:application/pdf File Journal:eJournal |
| author |
Prihatini, Istiana Rimbawanto, Anto Puspitasari, Desy Fauzi, Dayin |
| title |
PENGUJIAN PENANDA JENIS SPESIFIK PADA JAMUR YANG BERPOTENSI SEBAGAI AGENS PENGENDALI HAYATI PENYAKIT BUSUK AKAR PADA AKASIA |
| publisher |
Center for Forest Biotechnology and Tree Improvement (CFBTI) |
| publishDate |
2018 |
| topic |
DNA dilution Ganoderma PCR primers rapid identification |
| url |
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4276 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4276/4253 |
| contents |
Root rot caused by Ganoderma philippii (Bres. & Henn. ex Sacc.) Bres., is an important disease in Acacia plantation. A strategy to control this disease is currently being developed, particularly on the application of biological control agent (BCA). Species specific primers for rapid identification of potential fungi as BCA were developed. This study aimed to obtain the best DNA condition and the best primers for species specific identification. DNA with 20x dilution is the best condition for amplification of the ITS fragment thus used for rapid species identification. The best primer set to detect Cerrena sp. is CrF1/CrR1, PbF2/PbR2 is the best primer set for Phlebiopsis sp. 1. There was no specific primer suitable to detect Phlebia sp. 1 and Phlebia sp. 2 only but Pl-2F1/Pl-2R4 is the best primer set for Phlebia spp. |
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Kementerian Lingkungan Hidup dan Kehutanan Republik Indonesia |
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Perpustakaan Kementerian Lingkungan Hidup dan Kehutanan RI |
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Indonesian Journal of Forestry Research |
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Kehutanan Lingkungan Pertanian |
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BOGOR |
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JAWA BARAT |
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2018-06-19T23:35:44Z |
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2019-12-13T09:36:55Z |
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