Effect of Acid Pretreatment on Enzymatic Hydrolysis in Bioethanol Production from Rice Straw

Main Authors: Aditiya, H. B., Sing, K.P., Hanif, M., Mahlia, T.M.I.
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: Universitas Indonesia , 2015
Subjects:
Online Access: http://www.ijtech.eng.ui.ac.id/index.php/journal/article/view/784
http://www.ijtech.eng.ui.ac.id/index.php/journal/article/view/784/222
Daftar Isi:
  • Clean, safe and sustainable energy sources must be found to minimize all side-effects of fossil fuel consumption. Second generation bioethanol possesses a great potential as an alternative energy source especially in the transportation sector. In this study, rice straw was selected to be studied as a conversion of potential lignocellulosic biomass into bioethanol. Firstly, rice straw was processed with mechanical pretreatment using a home blender, followed by acid pretreatment using 2.0 M sulphuric acid (H2SO4) at 90oC for 60 minutes. The glucose yield was found to be 9.71 g/L. Then, rice straw pretreated with acid was hydrolyzed using 24 mg of cellulase from Tichoderma Ressei ATCC 26921 over a 72-hour duration, which yielded a total glucose count of 11.466 g/L. After fermentation with Saccharomyces cerevisiae, it was found that by combining enzymatic hydrolysis with acid pretreatment yielded a higher ethanol content after fermentation (0.1503% or 52.75% of theoretical value) compared to acidic pretreatment alone (0.013% or 11.26% of theoretical value).