ABILITY OF AMMONIUM EXCRETION, Indol Acetic Acid PRODUCTION, AND PHOSPHATE SOLUBILIZATION OF NITROGEN-FIXING BACTERIA ISOLATED FROM CROP RHIZOSPHERE AND THEIR EFFECT ON PLANT GROWTH
| Main Author: | Ali, Alimuddin |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Asian Research Publishing Network (ARPN)
, 2016
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| Subjects: | |
| Online Access: |
http://eprints.unm.ac.id/14849/1/1_z_ARPN%20Journal%20of%20Engeenering.pdf http://eprints.unm.ac.id/14849/2/1_z_ARPN%20%20Journal%20of%20Engeenering%20REVIEWER.pdf http://eprints.unm.ac.id/14849/3/1_z_ARPN%20Journal%20of%20Engeenering%20TURNITIN.pdf http://eprints.unm.ac.id/14849/ |
Daftar Isi:
- The aim of this research is to investigate the ability of nitrogen fixing bacteria isolates obtained from croprhizosphere in the District of Maros, South Sulawesi province, Indonesia to excrete ammonium, to produce growthpromoting substance called Indol Acetic Acid (IAA), to solubilize phosphate and their effect on plant growth. There were43 previously isolated nitrogen fixing bacterial isolates inoculated in Burk’s medium. Ammonium released was quantifiedusing spectrophotometry method. The isolates with the ability to excrete high amount of ammonium were selected to IAAproduction and phosphate solubilization analysis. IAA concentration was measured by spectrophotometry method andPhosphate solubilization assay was done by inoculating bacterial isolates on Pikovskaya medium. The ability to solubilizephosphate was marked by a clear zone around bacterial colonies. Selected isolates were then analysed for their effects ongrowth of Amaranthus tricolor L with pot trials conducted under green-house conditions. Among 43 nitrogen fixingbacterial isolates, 14 isolates could excrete ammonium in the concentration of 256, 7 μM until 1027, 77 μM. Those isolatesalso could produce IAA in the concentration between 5, 59 ppm to 41, 30 ppm. There were 6 isolates that could solubilizephospate with clear zone measure of 1, 5 cm to 3, 1 cm. From the pot trial to evaluate the effect of selected bacterial isolateto plant growth, it was found that inoculated plants have better growth than un-inoculated plants. In summary, all selectednitrogen fixing bacterial isolates in this study promoted the plant growth well. Keywords: ammonium excretion, IAA, nitrogen fixing bacteria, phosphate solubilisation, plant growth.