Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation
| Main Authors: | Pagarra, Halifah, Rahman, Roshanida A, Rachmawaty, Rachmawaty |
|---|---|
| Format: | Article NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Foundation for Enviromental Protection and Research
, 2019
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| Subjects: | |
| Online Access: |
http://eprints.unm.ac.id/14495/1/Halifah%2C%20biochemical-study-of-the-effect-of-nephrolepis-biserrata-pretreatment-on-exo-polygalacturonase-6193.pdf http://eprints.unm.ac.id/14495/2/t.%20combinepdf%20%20Biochemical%20%20Study.pdf http://eprints.unm.ac.id/14495/3/t.%20%20Turnitin_Biochemical%20study%20of%20the%20effect%20of%20Nephrolepis%20biserrata%20pretreatment%20on%20exo-polygalacturonase%20production.pdf http://eprints.unm.ac.id/14495/ http://www.ejobios.org/article/biochemical-study-of-the-effect-of-nephrolepis-biserrata-pretreatment-on-exo-polygalacturonase-6193 |
Daftar Isi:
- The substrate plays an important role in solid state fermentation (SSF) in the production of exo-polygalacturonase (one type of the pectinase enzymes). The aim of this study was to investigate a pretreatment method on Nephrolepis biserrata leaves as a substrate for the production of exo-polygalacturonase. The pretreatment steps improved the decomposition of the lignocellulosic material in the structure of N. biserrata leaves as a substrate and allowed Aspergillus niger better access of the substrate to the enzymatic reaction. This optimised both exo-polygalacturonase activity and the growth of A. niger in SSF. Pretreatment was performed under alkali, acidic, autohydrolytic and control conditions. The highest exo-polygalacturonase activity was 39.16 U/g and biomass of A. niger was 0.39 mg/gdsf at 120 h was found for the autohydrolysis pretreatment, as compared to alkali pretreatment, acid pretreatment and control. Scanning electron microscope images showed a marked change in the physical appearance of treated N. biserrata leaves as compared to untreated N. biserrata leaves, especially following autohydrolysis pretreatment.