THE QUALITY OF SEXED SEMEN ON FILIAL ONGOLE BULL USING PERCOLL DENSITY GRADIENT CENTRIFUGATION METHOD

Main Author: Enike Dwi Kusumawati, Enike
Format: Article PeerReviewed Book
Bahasa: eng
Subjects:
Online Access: http://repository.unikama.ac.id/2656/1/1.%20Asian%20Jr.%20of%20Microbiol.%20Biotech.%20Env.%20Sc.%20Vol.%2019%2C%20No.%20%281%29%20%202017%20%20189-199.pdf
http://repository.unikama.ac.id/2656/
Daftar Isi:
  • Abstract-Artificial Insemination technology can be increased in value by using sexed semen program that produce sex of calf as expected. The purpose of this study to determine the quality of sexed semen using percoll density gradien centrifugation method in Filial Ongole Cattle. Semen was collected using Artificial Vagina (AV) from Filial Ongole Cattle, evaluated and then separated using percoll density gradien centrifugation method (PDGC). Only fresh semen with a minimum of 70% individual motile sperm and 2+ mass of motility used in this study. Andromed as a based extender was diluted using aquabidest with 1:4 ratios. The obtained data were analyze with analysis of variance (ANOVA) and continued by Duncan test if there was significant or very significant different. The result showed that the semen quality of non-sexed sperms, upper (Y sperms) and under (X sperms) fraction using SGDP had very significant different (P<0,01) on motility (64,25±3,94%; 53±7,93%; 48,55±8,28% respectively), viability (95,07±0,99%; 88,64±0,51%; 85,15±0,84% respectively), abnormality (0,93±0,28%; 1,98±0,27%; 4,42±0,36% respectively). The study concludes that the sexed semen using percoll density gradien centrifugation method can be used as an alternative separation of X and Y sperms.