Uji Potensi dan Optimasi Konsentrasi Lignoselulosa Beberapa Limbah Pertanian sebagai Sumber Karbon dalam Produksi Enzim β-glukosidase Phanerochaete chrysosporium
| Main Author: | Wuri DAMAYANTI; Mahasiswa |
|---|---|
| Format: | PeerReviewed eJournal |
| Bahasa: | ind |
| Terbitan: |
SKRIPSI Jurusan Kimia - Fakultas MIPA UM
, 2014
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| Online Access: |
http://karya-ilmiah.um.ac.id/index.php/kimia/article/view/35929 |
Daftar Isi:
- ABSTRAK Damayanti, Wuri. 2014. Potential Test and Optimization Concentration of Lignocellulosic from Various Agricultural Waste as Carbon Source on Enzyme β-glucosidase Production from Phanerochaete chrysosporium. Undergraduated, Department of Chemistry, Faculty of Mathematics and Natural Science, State University of Malang. Advisors: (1) Evi Susanti, S.Si., M.Si., (2) Dr. Suharti, M.Si. Kata Kunci: β-glukosidase, , lignocellulosic, agricultural waste, carbon source, Phanerochaete chrysosporium Enzyme β-glucosidase is the enzyme that catalyzes hydrolysis reaction cellobiosa to glucose. Enzyme β-glucosidase production from P.chrysosporium is strongly influenced by the type of carbon source in the media production. The aimed of this researched were examine scientific information about (1) the most potential carbon source among lignocellulosic agricultural waste corn stover, bagasse, rice husk, rice straw and sawdust in β-glucosidase production from P.chrysosporium, (2) optimization concentration of the most potential lignocellulosic agricultural waste as carbon source in β-glucosidase production from P.chrysosporium This research are laboratory that conducted in Departmen of Chemistry, State University of Malang and Microbiology Laboratory, State University of Brawijaya Malang. The phase of this research are: (1) sample preparation (lignocellulosic) as carbon source are rice straw , rice husk , corn stover , bagasse and sawdust, (2) rejuvenation pure culture of P.chrysosporium, (3) manufacture of P.chrysosporium spore suspension, (4) β-glukosidase production from P.chrysosporium using lignocellulosic as carbon source, (5) determination the optimum concentration of the most potential carbon source to produce β-glukosidase enzyme from P.chrysosporium. Lignocellulosic concentration used are 0; 0,3; 0,5; 0,7; 1,0 dan 1,2 % (b/v). Selection the most potential carbon source and determination the optimum concentration based on the level of β-glucosidase activity. The β-glucosidase activity was determined by reduction of the amount of sugar produced after the reaction. Reducing sugar content was determined by using the DNS method. The result of this research showed that rice straw , rice husk , corn stover , bagasse and sawdust have the potential as carbon source on β-glucosidase production from P.chrysosporium. The use of waste corn stover , bagasse, rice straw, rice husk, and sawdust at a concentration of 1 % can produce consecutive the highest β-glucosidase activity 2,169 U/ mL, 4,853 U/ mL, 12,132 U/ mL, 5,00 U / mL and 6,691 U/ mL. The rice straw is the most potential lignocellulosic waste as carbon source on β-glukosidase production from P.chrysosporium. The rice straw can produce the highest β-glukosidase activity at 1% as big as 12,132 U/ mL.