Viability Testof Lemongrass Extract (Cymbopogon Citratus) on BHK-21 Fibroblast Cell Culture
| Main Authors: | Shafira Kurnia Supandi, Yoshua Christian Michael, Lambang Bargowo |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Institute of Medico-Legal Publications
, 2020
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/99600/1/3_Viability%20Testof%20Lemongrass%20Extract%20%28Cymbopogon%20Citratus%29.pdf http://repository.unair.ac.id/99600/2/3_Turnitin%2022%20Viability%20Testof%20Culture.pdf http://repository.unair.ac.id/99600/3/3_Viability%20test%20of%20Lemongtrass%20.....pdf http://repository.unair.ac.id/99600/ https://doi.org/10.37506/mlu.v20i2.1217 |
Daftar Isi:
- Background: Lemongrass are found in Indonesia thus it lemongrass can be used as an alternative in healing inflammation by conducting a preliminary study in the form of BHK-21 fibroblast cell viability test after 24 hours of extract lemongrass. Objective: To determine the viability of extract lemongrass against BHK-21 fibroblast cell cultures. Method: BHK-21 fibroblast cells were taken from neonatal hamsters’ kidneys and planted in 96 well plates. Extracts lemongrass with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.13% and 1.56% were included in each well prepared and incubated for 24 hours. MTT Assay was performed to see the viability of extract lemongrass on fibroblast cells. Results: Extract lemongrass viable against BHK-21 fibroblast cells at a concentration of 100%. There is a decrease in viability at concentrations of 50% to 1.56%. Conclusion: The 100% concentration showed the highest viability of BHK-21 fibroblast cells and extract lemongrass had potential in wound healing.