The Comparative Toxicity of Xanthones and Tannins in Mangosteen (Garcinia mangostana Linn.) Pericarp Extract against BHK‐21 Fibroblast Cell Culture
| Main Authors: | Fikarini H. Puteri, Jennifer Widjaja, Febriastuti Cahyani, Latief Mooduto, Dian Agustin Wahjuningrum |
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| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Wolters Kluwer - Medknow
, 2019
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/97322/2/Jurnal%20The%20Comparative%20Toxicity.pdf http://repository.unair.ac.id/97322/3/Turnitin%20The%20Comparative%20Toxicity.pdf http://repository.unair.ac.id/97322/1/validasi%20the%20comparative%20toxicity.pdf http://repository.unair.ac.id/97322/ |
Daftar Isi:
- Objective: The objective of this study is to compare the toxicity level of xanthones and tannins derived from mangosteen pericarp extract at specific concentrations against BHK‐21 fibroblast cell cultures. Methods: Mangosteen was extracted using a maceration method with ethanol 96%. Xanthones were isolated from the chloroform extract, whereas tannins were isolated using acetone alcohol and serial diluted to 100% concentration. Toxicity levels were monitored after 24 h using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide assay technique by ELISA reader at 620 nm. Results: Viable cells of BHK‐21 against xanthone concentration began to decrease (40.24%) at 3.98% xanthones, whereas viable cells of BHK‐21 against tannin concentration began to decrease (68.06%) at 2.2% tannins. Conclusion: It is suggested that tannins were more toxic than the xanthones derived from mangosteen pericarp.