Detection and Phylogenetic Analysis of Mycobacterium Leprae in Prehistoric Skull Bone From Lewoleba, Flores Island-Lembata Indonesia Based on NTT Regions
| Main Authors: | Eduardus Bimo Aksono Herupradoto, Koesbardiati, D. Adriaty, R. Wahyuni, Iswahyudi, I. Agusni, S. Izumi |
|---|---|
| Format: | Proceeding NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2013
|
| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/97057/1/proceeding%20the%20future%20of%20%20anatomy.2011.pdf http://repository.unair.ac.id/97057/ |
Daftar Isi:
- Introduction: Identification of the genetic material of pathogenic organisms in the prehistoric networks provide important information for the study of certain infectious diseases in prehistoric populations. In addition, the identification of bacterial DNA provides direct evidence and the frequency of occurrence of infectious diseases in prehistoric populations and may provide information about the evolution of microorganisms and related diseases. Several recent reports have succeeded in isolating several mycobacterium by using PCR technique, because the PCR technique, although very small amount of DNA in prehistoric biomaterials such as bone or soft tissue but can be identified. Objectives: To perform detection and phylogenetic analysis of M.leprae in prehistoric skull bone from Lewoleba Flores island-Lembata Indonesia based on TTC regions. Methods and Material: Lewo1eba site of origin of the skull bone, Lembata island Flores, Indonesia (code LL 115) which has been determined based on the C14 shows antikuitas age 2990 +/-160 BP. DNA extraction using Qiagen kit (and Proteinase-K for lysis). PCR run using primers LpF-R and Lpl,2 and Lpl,2-Lp3,4 (nested PCR). Purification and sequencing performed on 129 basepairs (bp) of PCR products, phylogenetic analysis based on TTC regions. Results: Swab the outside of the skull bones obtained one sample with 19 repetitions TTC and one sample with 14 repetitions TTC, whereas the inner bone obtained by 13 repetitions TTC. Conclusion: Isolates of M. /eprae has been identified from Lewoleba Flores island-Lembata Indonesia (code LL 1/5) with a PCR-based region of TTC. Generally seen that the same pattern obtained with TTC motifs derived from M. leprae isolates in Southeast Asia. Keywords: Skull-Bone, TTC regions, M. leprae, Prehistoric, PCR