Cloning of cDNA Encoding Membrane Protein of Tachyzoite of Toxoplasma gondii In pUC19
| Main Authors: | Mufasirin, Wayan T. Artama, Sumartono, Sri Hartati |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta
, 2015
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/95141/4/Bukti%2032%20Cloning%20of%20cDNA%20Encoding%20Membrane%20.....pdf http://repository.unair.ac.id/95141/2/Peer%20review%20C%2032.pdf http://repository.unair.ac.id/95141/ |
Daftar Isi:
- The aim of the research is to clone cDNA encoding membrane protein of Toxoplasma gondii using pUC19 for developing molecular diagnosis and vaccines. Total RNA and messenger RNA were isolated from tachyzoites of Toxoplasma gondii that growing up in Swiss strain mice using PolyAtract®mRNA Isolation Systems (Promega) and synthesis of cDNA using Riboclone®cDNA Synthesis Systems (Promega). Complementary DNA was ligated with EcoRI adaptor using Riboclone®EcoRIAdaptor Systems (Promega). preparation pUC19 vector, pUC19 ligated with cDNA was transformed in E. Coli DH5α. Plasmid recombinant was analyzed using endonuclease restriction enzyme (EcoRI and HindIII), total protein isolation and immunoblotting. Results of transformation using the pUC19 vector obtained two recombinant clones carrying genes encoding membrane proteins