Production and Characterization of Immunoglobuline Yolk as Anti Antigen Membrane Toxoplasma gondii
| Main Authors: | Yuliana Pratiwi, Heni Puspitasari, Lucia Tri Suwanti, Mufasirin |
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| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Institute of Tropical Disease University Airlangga
, 2016
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/95110/1/Bukti%20C%2012%20PRODUCTION%20AND%20CHARACTERIZATION%20OF%20I.....pdf http://repository.unair.ac.id/95110/2/Peer%20review%20C%2012.pdf http://repository.unair.ac.id/95110/ https://e-journal.unair.ac.id/IJTID/article/view/1365/2304 |
Daftar Isi:
- Toxoplasma gondii is an obligate parasite intracellular which can infection human and other mammalian. Immunoglobulin Y technology offers several advantages better than antibody production in mammals. This research is aimed to get immunoglobulin Y from egg yolk, and to find the characterization of immunoglobuline Y according to molecular weight by SDS PAGE and targeted protein with antibodies using Western Blot. This research divided from many step: culture tachyzoites of T. gondii fromintraperitoneal fluid, preparation of membrane antigen tachyzoite of T. gondii, then immunization laying hens with membrane antigen, extraction and purification immunoglobuline Y from egg yolk and then protein analyzed by SDS PAGE and Western Blot. The result of this research showed that immunoglobulin Y from egg yolk can produced antibody against protein membrane of T. gondiiandprofile protein immunoglobuline Y according SDS PAGE has molecular weight 179,8 kDa. Immunoglobuline Y was analyze by Western Blot can recognize antigen epitope of T.gondii on molecular weight 35,7kDa and 78,8 kDa. Keywords: Toxoplasma gondii, anti membrane T.gondii, immunoglobulin Y anti membrane