Identification of Toxoplasma gondii Excretory-Secretory Antigens from Propagation in Mice as Toxoplasmosis Vaccine Candidate
| Main Author: | Mufasirin |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Global Researchers Journal
, 2014
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/95100/5/Bukti%20C%2005%20identification%20of%20toxoplasma%20g....-dikompresi.pdf http://repository.unair.ac.id/95100/2/Peer%20review%20C%2005.pdf http://repository.unair.ac.id/95100/3/Turnitin%20Bukti%20C%2005%20Identification%20of%20Toxoplasma%20gondii%20....pdf http://repository.unair.ac.id/95100/ |
Daftar Isi:
- The aim of this research is to get some ESAs of T. gondii tachyzoite from propagation in mice and ESAs having the character of immunogenic as candidate of toxoplasmosis vaccine. The long-range target of this research is get subunit vaccine of toxoplasmosis. The methods used cover:propagation of T. gondii in mice and cropping ESAs at intraperitoneal fluid and fractination ESAs by SDS-PAGE, antibody making to ESAs, anddetermination antigenicity by western blot. The results of research founded 13 ESAs: 125.5; 100.9; 70.2; 56.7; 45.6; 40.4; 35.3; 34.1; 29.5; 27.4; 24.9; 20.7 and 19.7 kDa. ESAs having the character of antigenic were molecule weighing 100.9; 56.7; 35.3; 34.1; 24.9; 20.7 and 19.7 kDa, and 100.9; 56.7; 24.9; 20.7 and 19.7 kDa represent major antigenic. Keywords: Toxoplasma gondii, excretory-secretory antigens, vaccine