The Difference Between the Amount of Glucose as the Product of Metabolism of Glucosyltransferase Enzyme Streptococcus Mutans In Neutral pH and Optimal pH
Daftar Isi:
- Background:Caries happens due to the production of organic acid from the sugar metabolism, it cause a decrease in oral pH. The changes of pH affects the formation of glucan as the product of metabolism GTF enzyme S. mutans. Purpose: To investigate whether neutral pH (7) will decrease the amount of glucose compared to pH (5.5, 6.0, and 6,5). Methods: The S. mutans’s was cultured in BHI and centrifuged to obtain its supernatants. Six samples for each four group, consisted of 0.9 ml of 0.25 M sucrose in 0.2 M buffer, 0.1 ml of GTF enzyme, and buffer solution (pH 5.5, 6.0, 6.5, and 7) with total volume of 2ml (incubated), were then measured by using HPLC . Result: The amount of glucose (pH 7) are significantly less than samples (pH 5,5 and 6,5). Conclusion: The amount of glucose (pH 7) is less in acid pH, due to the changes of enzyme’s charge and denaturation.