Acceleration of wound healing with use of secretory leukocyte protease inhibitor could be seen by osteopontin expression in Rattus norvegicus post tooth extraction
| Main Authors: | D Fitriani, M.Cahyati, N Prasetyaningrum, Theresia Indah Budhy S., Erni Munadziroh |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
IOP Publishing
, 2018
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/91633/1/3.pdf http://repository.unair.ac.id/91633/2/scan%20tere%203.pdf http://repository.unair.ac.id/91633/3/Acceleration%20of%20wound%20healing%20with%20use%20of%20secretory%20leukocyte%20protease%20in%20hibitor%20could%20be%20seen%20by%20osteo%281%29.pdf http://repository.unair.ac.id/91633/ |
Daftar Isi:
- Tooth extraction can be debilitating due to prolonged inflammation delaying wound healing. Secretory leukocyte protease inhibitor (SLPI) is a non-glycosylated proteomic material that inhibits protease enzymes and has anti-inflammatory properties accelerating wound healing. Increased osteoclast activity is marked by increased osteopontin expression (OPN) during bone resorption. OPN is a phosphorylated glycoprotein secreted by several immune cells. This study aims to determine OPN’s expression as an osteoclast activity’s overview in wound healing acceleration, following SLPI administration. Thirty-six Rattus norvegicus incisors were extracted and randomly divided into 6 groups by treatment type and duration of observation. SLPI was administered in Group 1 and 2 (12 heads respectively) on the socket of rat’s teeth, followed by tight stitches. Group 2 additionally received bone graft. Control group K (12 heads) exclusively received tooth socket stitching. Two observations, each on 6 rats, were performed at 7 and 14 days. Subsequently, biopsies were performed and immunohistochemically stained with osteopontin antibodies. One-way Anova and Kruskal–Wallis tests showed statistically significant differences (p<0.05) between the groups. Results suggest that SLPI administration decreases osteoclast activity in alveolar bone resorption by decreasing the number of active osteoclasts and increasing the number of inactive ones in a Rattus model. Osteopontin expression can be used in overview of osteoclast’s activity.