ANALISIS HOMOLOGI DAN KARAKTERISTIK FILOGENETIK GEN PENGKODE PROTEIN FUSION (F) REGIO 4607-4718 BP VIRUS NEWCASTLE DISEASE PADA ISOLAT AYAM KAMPUNG (Gallus gallus domesticus) DI SURABAYA
| Main Author: | ASTARINA WILI MARTHA, 061511133272 |
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| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | ind |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/88943/1/KKC%20KK%20KH%20157-19%20Mar%20a-Abstrak.pdf http://repository.unair.ac.id/88943/2/KKC%20KK%20KH%20157-19%20Mar%20a-Daftar%20isi.pdf http://repository.unair.ac.id/88943/3/KKC%20KK%20KH%20157-19%20Mar%20a-Daftar%20pustaka.pdf http://repository.unair.ac.id/88943/4/KKC%20KK%20KH%20157-19%20Mar%20a.pdf http://repository.unair.ac.id/88943/ http://lib.unair.ac.id |
Daftar Isi:
- Newcastle Disease caused by Newcastle Disease Virus genus avulavirus, family Paramyxoviridae, which had seven genetic code protein. One of them is Fusion (F) protein. Fusion (F) protein play an important role to enveloped virus with membrane tissue infection. Therefore, this study was to analyze the homology of nucleotide sequence, homology percentage, and determine of phylogenetic relation from fusion (F) protein coding gene of Newcastle Disease (ND) from Bantam Chicken (Gallus gallus domesticus) in Surabaya comparison isolate from GenBank. Sample have three of thirty-six samples isolated from domestic avian in Surabaya were positive ND. One of these samples were Bantam Chicken (Gallus gallus domesiticus) sample. Titre of HA and HI the Bantam Chicken (Gallus gallus domesticus) show 27. Then, sample were identyfied using One Step RT-PCR with primer forward and reverse with target 112 bp. The sequence of PCR product was analyse using BioEdit ver.7.0. The result of homology was analyzed using Needle-Wunsch Global Align Nucleotide Sequence on NCBI. These score 88% were compared with LaSota strain and show 85% with Komarov strain. The score 68-71% were compared to reference sequences from GenBank. The conclusion was Bantam Chicken has quite high similarity with LaSota strain and Komarov strain and Phylogenetic analysis from isolates in this research relate to more isolates of vaccine and more isolates in Indonesia.