The Effect of Estrogen on Type 2 Collagen Levels in the Joint Cartilage of Post-Menopausal Murine Subjects
| Main Authors: | Bimo Sasono, Fedik Abdul Rantam, NIDN. 0010035907, Heri Suroto, NIDN. 8802630017, Hari Basuki Notobroto, NIDN. 0025066504 |
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| Format: | Article PeerReviewed Book |
| Bahasa: | ind |
| Terbitan: |
The Hard Tissue Biology Network Association
, 2019
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/86493/1/PAK%20HARI.pdf http://repository.unair.ac.id/86493/ https://www.jstage.jst.go.jp/article/jhtb/28/3/28_245/_article/-char/ja/ https://doi.org/10.2485/jhtb.28.245 |
Daftar Isi:
- Cartilage degradation frequently occurs during the postmenopausal period due to a decrease in type 2 collagen and constitutes a condition affected by the level of estrogen in the body. The purpose of this study is to prove that such post-menopausal reductions in estrogen levels influence the type 2 collagen expression in cartilage. Oophorectomies were per-formed on 54 Rattus norvegicus subjects and each of which was subsequently assigned to one of six different groups. 17β-estradiol present in the blood of the subjects was examined by means of ELISA technique, while the expression of MMP-13 and type 2 collagen in their knee joint cartilage was assessed immunohistochemically. A significant post-oopho-rectomy decrease of 17β-estradiol was detected in subjects (p<0.05), while the expression of MMP-13 increased apprecia-bly (p<0.05) and a marked decrease in type 2 collagen expression occurred (p<0.05). The reduction in 17β-estradiol pro-moted an increase in the expression of MMP-13 and a decrease in type 2 collagen expression.