Phase Behavior of Dried – DDA Liposomal Formulation Dispersed in HPMC Matrix in the presence of Saccharides
| Main Authors: | Helmy Yusuf, NIDN. 0015077901, Raditya Nugraheni, Nur Aini Mulyadi, Dwi Setyawan, NIDN. 0030117104 |
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| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
International Journal of Pharm Tech Research
, 2017
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/85788/6/C-16.pdf http://repository.unair.ac.id/85788/2/Phase%20Behavior%20of%20Dried%20%E2%80%93%20DDA%20Liposomal%20Formulation%20Dispersed%20in%20HPMC%20Matrix%20in%20the%20presence%20of%20Saccharides.pdf http://repository.unair.ac.id/85788/5/C-16%20Rev.pdf http://repository.unair.ac.id/85788/ http://sphinxsai.com/2017/ph_vol10_no1/ph01.htm |
Daftar Isi:
- The present study describes the effect of saccharides and hydroxypropyl methylcellulose (HPMC) matrix on phase behavior of dehydrated cationic dimethyl-dioctadecylammonium (DDA)-based liposomes. Saccharides such as sucrose, lactose and mannitol,have been reportedpreserve the lipid membranes during drying, whilst HPMC matrix is widely used in solid dispersion to prevent aggregation and/or recrystallization.The study revealed that addition of sucrose and HPMCin the formulation demonstrated a miscible mixture that might construct a stable dried liposomal formulation. DTA data showed that sucrose (5%w/v) and HPMC added to DDA liposomal formulation were relatively more miscible with the mixtures; whereas lactose and mannitol at the same concentration of 5% showed phase separation from the mixtures in the dehydrated state. Furthermore, XRD and SEM analysis exhibited supporting evidences in which formulation using sucrose and lactose showedrelatively less crystalline-forming properties compared to formulation using mannitol. Recrystallization that cause phase separation might trigger leakage and further affect the efficacy of the entrapped drug/antigen. From these data, it might be concluded that a driedliposomal formulation can be prepared in the presence of sucrose (lyoprotectant) that is dispersed in HPMC matrix. The protective mechanism of sucrose (5%w/v) and HPMC matrix is proposed through inhibition of the recrystallization which causes phase separation; indicated by DTA, SEM and XRDdata. The present study revealed prospective advantages of using sucrose and HPMC in development of dried – DDA liposomal formulations.