Pengaruh Elisitor Ekstrak Saccharomyces cerevisiae terhadap Biomassa dan Kadar Saponin Akar Adventif Talinum paniculatum (Jacq) Gaertn., Secara In-vitro
Daftar Isi:
- ABSTRACT This study aimed to determine the effect of various concentrations of S. cerevisiae extract as biotic elicitor and time of harvesting to biomass and saponin levels of adventitious roots T. paniculatum(Jacq) Gaertn in-vitro. Leaves of T. paniculatum grown on Murashige and Skoog medium (MS) supplemented 2 mg /L IBA and S. cerevisiae extract with concentrations of 0; 0.025; 0.05, and 0.1% g / L. Biomass and saponin levels of adventitious root T. paniculatum measured at harvest age 2, 4, and 6 weeks. Biomass measured after fresh weight of adventitious roots T. paniculatum dried in the oven 500C, for 5 days. Saponin levels are determined by semi-quantitative method on TLC plate and eluted with propanol : H2O with a ratio of 14 : 3. Elicitor concentration 0.025% by harvest time at the 4th week produced highest average biomass of 16.1 mg. Lowest mean value of biomass produced at giving elicitor concentration 0.1% harvesting the 2nd week in 1.4 mg. Brown-Forsythe test results show extracts of S. cerevisiae give effect on adventitious root biomass T. paniculatum (Sig 0.003 <α 0,05). Significant difference treatments Only shown in giving elicitor concentration of 0.1% (sig 0.011 <α 0,05). Harvesting time give effect on biomass adventitious root T. paniculatum (Sig 0.043 <α 0.05), From Games-Howel test showed no significant difference between treatments. The best concentration of S. cerevisiae extract is to increase of saponin contetns in adventitious root of T. paniculatum is 0.025% of 2 weeks culture.