Induksi Regenerasi Phalaenopsis amabilis (L.) Bl pada Berbagai Dosis Zat Pengatur Tumbuh
| Main Authors: | Edy Setiti Wida Utami, Sucipto Hariyanto |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) Universtas Tadulako
, 2015
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/83556/1/C%2017.%20J.%20of%20Nat%20Sciences%204%20%282%29%3B%2078-89%20%28%20Agustus%202015%29.%20ISSN%202338-0950.%20%28Ketua%29.pdf http://repository.unair.ac.id/83556/2/C%2017.%20J.%20of%20Nat%20Sciences%204%20%282%29%3B%2078-89%20%28%20Agustus%202015%29.%20ISSN%202338-0950.%20%28Ketua%29.pdf http://repository.unair.ac.id/83556/19/C17.%20Similarity.pdf http://repository.unair.ac.id/83556/20/C%2017.%20Validasi.pdf http://repository.unair.ac.id/83556/ http://jurnal.untad.ac.id/jurnal/index.php/ejurnalfmipa/article/view/4484 |
Daftar Isi:
- ABSTRACT The research was aimed to study the effect of plant growth regulator 2iP, NAA and its combination to regeneration of P. amabilis. Internodes and nodes segments from pedicellus were cultured in New Phalaenopsis (NP) media supplemented either with 2iP or NAA and its combination with concentrations of 0.0:0.0; 0.0:2.5; 0.5:2.0; 1.0:1.5; 1.5:1.0; 2.0:0.5, and 2.5:0.0 mg L-1 respectively. Eksplans were sub-cultured in the same medium once four weeks during twelve weeks. Callus initiation time was observed once a day. Shoot initiation time from internodes and nodes were observed once a week. The number of shoot and root were calculated twelve weeks after inoculation. The results showed that bud regeneration from internodes was indirectly due to callus phase, while bud and root regeneration from nodes were directly without callus phase. The combination between 2iP and NAA had higher effect on regeneration than single or without plant growth regulator. Nodes of explants produced more shoots and roots than internodes of explants.