PENGARUH DEMINERALIZED FREEZE DRIED BOVINE BONE XENOGRAFT TERHADAP EKSPRESI PROTEIN RUNT- RELATED TRANSCRIPTION FACTOR-2 DAN PROTEIN ALKALINE PHOSPHATASE PADA KULTUR HUMAN ADIPOSE DERIVED MESENCHYMAL STEM CELL ( Penelitian Eksperimental Laboratorik In Vitro )
| Main Author: | I KOMANG SUTRIADI TEGUH KELANA PUTRA NUGRAHA, 021218016301 |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | ind |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/81209/1/TKKli.%2015-19%20Nug%20p%20Abstrak.pdf http://repository.unair.ac.id/81209/2/TKKli.%2015-19%20Nug%20p.pdf http://repository.unair.ac.id/81209/ http://lib.unair.ac.id |
Daftar Isi:
- Background: One source of non-immunogenic bone graft is Bovine Hydroxyapatite (BHA), but it is lacking in the potential for osteoinduction. Recent research has shown that demineralized freeze-dried bovine xenograft (DFDBX) has the potential for osteoinduction and contains transforming growth factor β (TGF β) and Bone Morphogenic Protein (BMP) which trigger differentiation of Bone Marrow Messenchymal (BM-MSC) into osteoblasts. This study was conducted to assess the potential for osteoinduction in vitro using human adipose derived mesenchymal stem cells (hAD-MSC) by comparing proteins that express RUNX-2 and ALP on BHA that have been used and commercialized compared to new materials developed by DFDBBX. Objective: to determine the expression of RUNX-2 protein and ALP protein in hAD-MSC cultures after exposure to DFDBBX and BHA. Method: hAD-MSC cultures were divided into 4 groups which were 2.5% DFDBBX conditioned medium, 2.5% BHA conditioned medium, positive control (osteogenic medium), and negative control group (containing the basic medium of cell growth) and then planted in slide on the microplate. Observation of RUNX-2 and ALP expression was carried out after 2, 7 and 14 days of culture with the western blot methods. Result: The results of the study carried out using western blot examination and ImageJ software showed that there were differences in the number of RUNX-2 and ALP expressions on days 2, 7 and 14. Conclusion: Through this research it can be proven that there are expressions of RUNX-2 protein and ALP protein in Human adipose mesenchymal stem cell cultures after exposure to DFDBBX and BHA with Western blot examination methods.