Effect of hematopoietic stem cell on tumor necrosis factor-α expression, spiral artery remodeling and placental apoptosis in lead-exposed pregnant mice
Daftar Isi:
- Objective: To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF- a expression, spiral artery remodeling, and placental apoptosis. Methods: The study used pregnant mice divided to five groups of treatment: Group 1, without treatment; Group 2, 1/12 LD50 of lead and Aquadest; Group 3, 1/16 1-DSO of lead and Aquadest, Group 4, 1/12 LDso of lead and 1 X 107 HSC; and group 5, 1/16 1-DSO of lead and 1 X 107 HSC. Histopathology examination was performed to identify spiral artery remodeling. Immunohistochemistry analysis was conducted to characterize TNF- a expression and the apoptosis index. The data from all five groups were compared using the ANOVA test. Results: TNF- a expression differed significantly among the groups that were not exposed to lead, exposed to lead and exposed to lead after HSC treatment (P<O.OI). There was also a significant difference of spiral artery remodeling between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment (P<0.05). In apoptosis index evaluation there was a significant difference between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment, but not in the gimps of 1/16 1-DSO of lead followed HSC (P>0.05). Conclusions: In lead-exposed pregnant mice, the administration of hematopoietic stem cell may decrease TNF- a expression, spiral artery remodeling, and placental apoptosis index but in the group of I /1 6 LDs, of lead and HSC treatment, apoptosis index has no differences with those of control group