DETEKSI GEN blaTEM SEBAGAI PENYANDI Extended Spectrum Beta-lactamase PADA BAKTERI Klebsiella pneumoniae YANG DIISOLASI DARI SWAB HEWAN PENGHASIL PANGAN PENELITIAN EKSPLORATIF LABORATORIS
| Main Author: | INTAN GALUH BINTARI, 061614253004 |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | ind |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/70661/1/TPKMV.%2004-18%20Bin%20d%20Abstrak.pdf http://repository.unair.ac.id/70661/2/TPKMV.%2004-18%20Bin%20d.pdf http://repository.unair.ac.id/70661/ http://lib.unair.ac.id |
Daftar Isi:
- Klebsiella pneumoniae is one of nine bacteria in resistance to antibiotics in concern. This research aims to detect any gene of blaTEM in bacteria of the Klebsiella pneumoniae isolated from swab of food-producing animals. In this study, 195 swab samples taken from 17 locations of sampling. A cultivated sample obtained on selective medium and has done through the test identification, antibiotic sensitivity test method using Kirby-Bauer of agar diffusion against antibiotics of Ampicillin, Cefotaxime, Amoxcilin, Meropenem and Trimetrophrim-Sulfamethoxazole and afterwards tested with PCR method for detecting the gene it is causing antibiotic resistance. The results showed that 195 samples were found positive of Klebsiella pneumoniae isolates by as much as 10 sample i.e., 4 samples of dairy cows (SP-S1, SP-S3, SP-B2, SP-G4), two samples of beef cattle (SPT-K1, SPT-K2), 1 chicken samples (A-W5) and 3 samples of fish (IN-P2, IN-P3, IN-S3). Ten positive isolates were found indicating the resistant to Amoxycillin as much as 90% (9/10) and PCR tests also showed 9 sample (90%) have a positive gene blaTEM. Nine samples of the whole of the positive samples were resistant to the antibiotic Amoxicillin, i.e., SP-S3, SP-B2, SP-G4, SPT-K1, SPT-K2, A-W5, IN-P2, IN-P3, and the IN-S3. Based on the results of this research, with the discovery of antimicrobial agent resistance i.e. ESBL genes of encoder (blaTEM), researchers believe that the vast majority of isolates Klebsiella pneumoniae producing food animals have had ESBL encoding gene.