ANALISIS SEKUENS NUKLEOTIDA GEN 16S rRNA Mycobacterium tuberculosis Complex (MTBC) DARI SPUTUM PASIEN TB PARU
| Main Author: | NASTITI INTAN PERMATA SARI, 011514253008 |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2017
|
| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/67103/1/abstrak.pdf http://repository.unair.ac.id/67103/2/NASTITI%20INTAN%20PERMATA%20SARI_IKT_FK%20UNAIR_TESIS_2017.compressed.pdf http://repository.unair.ac.id/67103/ |
| ctrlnum |
67103 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>http://repository.unair.ac.id/67103/</relation><title>ANALISIS SEKUENS NUKLEOTIDA GEN 16S rRNA Mycobacterium
tuberculosis Complex (MTBC) DARI SPUTUM PASIEN TB PARU</title><creator>NASTITI INTAN PERMATA SARI, 011514253008</creator><subject>R Medicine</subject><description>Tuberculosis is infectious disease which was caused by the member of
pathogenic bacteria group Mycobacterium tuberculosis Complex (MTBC). This
study was aimed to analyze nucleotide sequence of 16S rRNA gene from MTBC
group isolated in pulmonary tuberculosis patient sputums. Sequencing results was
compared to sequences of Mycobacterium tuberculosis strain H37Rv (wildtype)
to determine species homology. MTBC species homology hopefully could be
used as information for the benefit of pulmonary tuberculosis molecular
epidemiology.
This study used sputum samples of pulmonary TB patients from TB clinic,
Dr. Soetomo General Hospital, Surabaya collected from September to November
2016, amounted up to 96 samples. Methods used includes smear BTA staining,
microbe culture, rapid test, and molecular method, such as 16S rRNA gene
amplification as used in this study to determine bacterial nucleotide sequences
from pulmonary TB, in order to analyze bacterial homology to different virulent
strain of Mycobacterium tuberculosis (strain H37Rv).
The samples with positive PCR and positive culture results were
proceeded to the next stage of sequencing. Sequences obtained from genotyping
16S rRNA gene in sputum of pulmonary TB had 70-76% homology to the MTBC
strains gene from the Gene Bank. Percentage was caused by considerable
variation in the regions suspected as the variable region.
Analysis of Mycobacterium tuberculosis Complex using 16S rRNA gene
target could not be used yet as basis for determining MTBC group but this gene
could be used for identification or phylogenetic analysis and nucleotides variation
in variable region</description><date>2017-11-22</date><type>Thesis:Thesis</type><type>PeerReview:NonPeerReviewed</type><type>Book:Book</type><language>eng</language><identifier>http://repository.unair.ac.id/67103/1/abstrak.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://repository.unair.ac.id/67103/2/NASTITI%20INTAN%20PERMATA%20SARI_IKT_FK%20UNAIR_TESIS_2017.compressed.pdf</identifier><identifier> NASTITI INTAN PERMATA SARI, 011514253008 (2017) ANALISIS SEKUENS NUKLEOTIDA GEN 16S rRNA Mycobacterium tuberculosis Complex (MTBC) DARI SPUTUM PASIEN TB PARU. Thesis thesis, Universitas Airlangga. </identifier><recordID>67103</recordID></dc>
|
| language |
eng |
| format |
Thesis:Thesis Thesis PeerReview:NonPeerReviewed PeerReview Book:Book Book |
| author |
NASTITI INTAN PERMATA SARI, 011514253008 |
| title |
ANALISIS SEKUENS NUKLEOTIDA GEN 16S rRNA Mycobacterium
tuberculosis Complex (MTBC) DARI SPUTUM PASIEN TB PARU |
| publishDate |
2017 |
| isbn |
011514253008 |
| topic |
R Medicine |
| url |
http://repository.unair.ac.id/67103/1/abstrak.pdf http://repository.unair.ac.id/67103/2/NASTITI%20INTAN%20PERMATA%20SARI_IKT_FK%20UNAIR_TESIS_2017.compressed.pdf http://repository.unair.ac.id/67103/ |
| contents |
Tuberculosis is infectious disease which was caused by the member of
pathogenic bacteria group Mycobacterium tuberculosis Complex (MTBC). This
study was aimed to analyze nucleotide sequence of 16S rRNA gene from MTBC
group isolated in pulmonary tuberculosis patient sputums. Sequencing results was
compared to sequences of Mycobacterium tuberculosis strain H37Rv (wildtype)
to determine species homology. MTBC species homology hopefully could be
used as information for the benefit of pulmonary tuberculosis molecular
epidemiology.
This study used sputum samples of pulmonary TB patients from TB clinic,
Dr. Soetomo General Hospital, Surabaya collected from September to November
2016, amounted up to 96 samples. Methods used includes smear BTA staining,
microbe culture, rapid test, and molecular method, such as 16S rRNA gene
amplification as used in this study to determine bacterial nucleotide sequences
from pulmonary TB, in order to analyze bacterial homology to different virulent
strain of Mycobacterium tuberculosis (strain H37Rv).
The samples with positive PCR and positive culture results were
proceeded to the next stage of sequencing. Sequences obtained from genotyping
16S rRNA gene in sputum of pulmonary TB had 70-76% homology to the MTBC
strains gene from the Gene Bank. Percentage was caused by considerable
variation in the regions suspected as the variable region.
Analysis of Mycobacterium tuberculosis Complex using 16S rRNA gene
target could not be used yet as basis for determining MTBC group but this gene
could be used for identification or phylogenetic analysis and nucleotides variation
in variable region |
| id |
IOS3215.67103 |
| institution |
Universitas Airlangga |
| institution_id |
33 |
| institution_type |
library:university library |
| library |
Perpustakaan Universitas Airlangga |
| library_id |
468 |
| collection |
Airlangga Institutional Repositories |
| repository_id |
3215 |
| subject_area |
Adat Istiadat Administrasi Negara dan Militer Agama |
| city |
KOTA SURABAYA |
| province |
JAWA TIMUR |
| repoId |
IOS3215 |
| first_indexed |
2017-11-30T22:53:28Z |
| last_indexed |
2017-12-19T23:10:16Z |
| recordtype |
dc |
| _version_ |
1766060117111865344 |
| score |
17.538404 |