Curcumin Menghambat Karbonilasi Protein Lensa pada Model Tikus Diabetes yang Diinduksi Streptozotocin
| Main Authors: | Meilia, Wulandari, Masyithoh Budi, Dewi, Ditya Ayu Intan, Setiowati, Duhita Pramesti, Hayuningtyas, Kartika Afrida, Fauzia, Purwanto, Bambang |
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| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Ikatan Ahli Ilmu Faal Indonesia (IAIFI)
, 2014
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/60150/1/karil12.Curcumin_Menghambat.pdf http://repository.unair.ac.id/60150/2/Peerreview12.CurcuminMenghambat.pdf http://repository.unair.ac.id/60150/ http://beasiswa.unair.ac.id/sites/default/files/perpanjangan/2013/ki_Bambang%20Purwanto_4662.pdf |
Daftar Isi:
- Aim : lens protein carbonylation is one of mechanism th at involved in complication cataract of diabetes. T he aim of this study was proving the effect of curcumin antio xidant to inhibit lens protein carbonylation in dia betic mouse models. Material and Method: curcumin was solved in maize (corn) oil with concen tration 0.4 mL/100 mg curcumin. Diabetic mouse models were induced by intraperitone al Streptozotocin injection with dose 150 mg/kg of body weight. Curcumin was given once a day as long as two days t o the mouse that had glucose level more than 200 mg /dL. The effect of curcumin was compared to control group, a qua treated group, metformin group and curcumin+met formin group. Lens of diabetic mouse models were pounded t o get homogenate that will be examined the carbonyl protein level. Absorbance was read using spectrophotometer with wave length 375 nm. Result and Discussion: Carbonyl protein levels (nm/ml) were like below: Control gro up (33.16± 19.83), Diabetes+Curcumin group (32.07± 9. 52), Diabetes+Metformin (98.61± 17.72), Diabetes+Curcumin +Metformin group (57.27± 12.19), Diabetes+aquades gr oup (311.83± 73.69). Carbonyl protein level of diabetic mouse lens that given curcumin was not different wi th control group and curcumin+metformin group. Carbonyl protein leve l of diabetic mouse lens that given curcumin was si gnificantly different with aqua treated group and metformin gro up. Conclusion: curcumin inhibits carbonylation of lens protein in diabetic mouse models that induced by streptozotoci n. Suggestion: this experiment must be improved more in order to give option preventive therapy of cataract compl ication of diabetes.