The Role of p53 Protein Expression in Retinoblastoma
| Main Authors: | Soebagjo, Hendrian Dwikoloso, Fatmariyanti, Susy, Lutfi, Delfitri, Aulanni’am, , Sumitro, Sutiman Bambang |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
European Journal of Biomedical and Phamnceutical Sciences
, 2016
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| Subjects: | |
| Online Access: |
http://repository.unair.ac.id/52998/2/Karil7%20The%20Role%20of%20p53oke.pdf http://repository.unair.ac.id/52998/3/Peereview7%20The%20Role%20of%20p5318022017122804.pdf http://repository.unair.ac.id/52998/14/article_ejbps_volume_3_july_issue_7_1467262157.pdf http://repository.unair.ac.id/52998/ http://www.ejbps.com/admin/download/article/dm9sdW1lXzNfanVseV9pc3N1ZV83LzE0NjcyNjIxNTcucGRm |
Daftar Isi:
- This study was undertaken to demonstrate the expression of p53 in retinoblastoma. Thirty three retinoblastoma tissue samples of intratumoral retinoblastoma were tested with SDS-PAGE to find the expression of retinoblastoma protein, followed by Western blotting and immunohistochemistry to confirm p53 expression. The electrophoresis examination identified 10 major protein fractions with molecular weight from 103 – 14 kDa. Poorly differentiated retinoblastoma samples revealed 10 protein fractions (molecular weight 103 – 14 kDa) and well differentiated retinoblastoma revealed 9 protein fractions (molecular weight from 97 – 14 kDa). Western blot and immunohistochemistry assay using monoclonal antibody p53 showed that samples are positively expressed p53. No significant correlation was found between p53 expression and differentiation of retinoblastoma (p<0.429, C = 0,214).